PF3D7_0507700 (NPL4)

Genome location: Pf3D7_05_v3:316,610..319,032(-)

Genome classification: Core

Function and Localization

Product Description: nuclear protein localization protein 4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0043130	ubiquitin binding
Function	GO:0031625	ubiquitin protein ligase binding
Process	GO:0030433	ubiquitin-dependent ERAD pathway
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.35	0.83
Trophozoite	expressed	1.04	0.47
Schizont	expressed	0.37	0.77
Gametocyte	expressed	0.85	0.54

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0507700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.935 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1107300)

Relative Growth Rate: 0.38 ± 0.32
Confidence: 3.69

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I426

"Best" AlphaFill ligand hit: BCN (bicine, Local RMSD=0.05) with 6JWI (Global RMSD=3.71)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101880

Most Similar Human Ortholog: I3L4U9

TM-align score: 0.62 | RMSD: 3.27

Seq Identity: 0.14 | Length: 107 / 531

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000182446	NPL4 homolog, ubiquitin recognition factor

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	23	20	30
disruptive	3	20	23	56
missense	3	19	21	49

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	62	26	33
disruptive	8	84	34	97
missense	7	70	34	76

PlasmoDB Total SNPs: 58

Non-coding: 41 | Synonymous: 13 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 531 | Molecular Weight (kDa): 62.575

UniProt ID(s): Q8I426

PDB ID(s): None

Isoelectric Point: 6.89

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000626	Ubiquitin-like domain
InterPro	IPR007716	NPL4, zinc-binding putative
InterPro	IPR007717	Nuclear pore localisation protein NPL4, C-terminal
InterPro	IPR016563	Nuclear protein localization protein 4
InterPro	IPR024682	Nuclear pore localisation protein Npl4, ubiquitin-like domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
PFam	PF05020	NPL4, zinc-binding putative
PFam	PF05021	Nuclear pore localisation protein NPL4, C-terminal
PFam	PF11543	Nuclear pore localisation protein Npl4, ubiquitin-like domain
Superfamily	SSF54236	Ubiquitin-like domain superfamily