Product Description: ceramide synthase CERS1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 7
EC Numbers: 2.3.1.24 (Sphingosine N-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0020011 | apicoplast |
| Component | GO:0009986 | cell surface |
| Component | GO:0005783 | endoplasmic reticulum |
| Function | GO:0016410 | N-acyltransferase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.08 | 0.95 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.24 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0508200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.394 | MFS: -2.571 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1107800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1107800)
Modification: Disrupted | RMgm-2960
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4D2
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.24 | sphingosine N-acyltransferase | FTY720SP600125fumonisinTriton X-100fumonisin B1Fumonisin B24,4'-diisothiocyanostilbene-2,2'-disulfonic acid3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100372
Most Similar Human Ortholog: Q6ZMG9
TM-align score: 0.72 | RMSD: 2.97
Seq Identity: 0.18 | Length: 283 / 355
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000090661 | ceramide synthase 4 |
| ENSG00000139624 | ceramide synthase 5 |
| ENSG00000143418 | ceramide synthase 2 |
| ENSG00000172292 | ceramide synthase 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 1 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 154
Non-coding: 142 | Synonymous: 11 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 355 | Molecular Weight (kDa): 42.503
UniProt ID(s): C0H4D2
PDB ID(s): None
Isoelectric Point: 9.6
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006634 | TRAM/LAG1/CLN8 homology domain |
| InterPro | IPR016439 | Sphingosine N-acyltransferase Lag1/Lac1-like |
| PFam | PF03798 | TRAM/LAG1/CLN8 homology domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |