PF3D7_0508400

Genome location: Pf3D7_05_v3:344,931..346,744(+)

Genome classification: Core

Function and Localization

Product Description: CFA20 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0036064	ciliary basal body
Component	GO:0031514	motile cilium
Component	GO:0000126	transcription factor TFIIIB complex
Process	GO:0060271	cilium assembly
Process	GO:2000147	positive regulation of cell motility
Process	GO:0060296	regulation of cilium beat frequency involved in ciliary motility
Process	GO:0006366	transcription by RNA polymerase II

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.02	0.99
Schizont	expressed	0.02	0.98
Gametocyte	expressed	0.26	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0508400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.194 | MFS: -2.594 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1108000)

Relative Growth Rate: 0.98 ± 0.12
Confidence: 5.59

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1108000)

Modification: Disrupted | RMgm-2961

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I419

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102689

Most Similar Human Ortholog: Q9Y6A4

TM-align score: 0.94 | RMSD: 1.70

Seq Identity: 0.47 | Length: 190 / 366

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000070761	cilia and flagella associated protein 20

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	21	6	23
disruptive	8	36	24	50
missense	6	27	20	45

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	41	14	20
disruptive	17	78	31	82
missense	11	57	25	56

PlasmoDB Total SNPs: 103

Non-coding: 65 | Synonymous: 28 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 366 | Molecular Weight (kDa): 43.09

UniProt ID(s): Q8I419

PDB ID(s): None

Isoelectric Point: 4.68

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007714	CFA20 domain
InterPro	IPR040441	Cilia- and flagella-associated protein 20/CFAP20DC
PFam	PF05018	CFA20 domain
Superfamily	N/A	N/A