About Gene List

PF3D7_0508700 (PRP5)

Genome location: Pf3D7_05_v3:358,035..362,825(+)

Genome classification: Core

Function and Localization

Product Description: pre-mRNA-processing ATP-dependent RNA helicase PRP5, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus
Function GO:0008186 ATP-dependent activity, acting on RNA
Function GO:0003724 RNA helicase activity
Process GO:0000398 mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.67 0.70
Trophozoite possibly expressed 1.08 0.46
Schizont possibly expressed 0.58 0.67
Gametocyte expressed 1.29 0.38

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0508700

Resistome Missense Mutations: E421K

Resistome Compounds with Missense Mutations: BI-2536

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.181 | MFS: -2.754 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1108300)

  • Relative Growth Rate: 0.39 ± 0.39
  • Confidence: 3.29

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I416

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.01) with 4KBF (Global RMSD=1.97)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.4.13 RNA helicase ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102375

Most Similar Human Ortholog: A0A0C4DG89

TM-align score: 0.52 | RMSD: 4.89

Seq Identity: 0.36 | Length: 593 / 1490

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000145833 DEAD-box helicase 46

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 18 70 36 77
disruptive 47 143 97 242
missense 31 125 90 192

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 26 137 53 97
disruptive 83 350 177 393
missense 49 284 138 237

PlasmoDB Total SNPs: 218

Non-coding: 62 | Synonymous: 85 | Nonsynonymous: 71 | Stop Codon: 0

Protein Information

Protein Length: 1490 | Molecular Weight (kDa): 175.807

UniProt ID(s): Q8I416

PDB ID(s): None

Isoelectric Point: 9.18

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000629 ATP-dependent RNA helicase DEAD-box, conserved site
InterPro IPR001650 Helicase, C-terminal
InterPro IPR011545 DEAD/DEAH box helicase domain
InterPro IPR014001 Helicase superfamily 1/2, ATP-binding domain
InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFam PF00270 DEAD/DEAH box helicase domain
PFam PF00271 Helicase, C-terminal
Superfamily SSF52540 P-loop containing nucleoside triphosphate hydrolase

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
31666190 Stress-induced upregulation of the ubiquitin-relative Hub1 modulates pre-mRNAsplicing and facilitates cadmium tolerance in Saccharomyces cerevisiae Chanarat S, Svasti J 10.1016/j.bbamcr.2019.118565
32320410 Impact of cancer-associated mutations in Hsh155/SF3b1 HEAT repeats 9-12 onpre-mRNA splicing in Saccharomyces cerevisiae Kaur H, Groubert B, ..., Hoskins AA 10.1371/journal.pone.0229315
31110137 Cus2 enforces the first ATP-dependent step of splicing by binding to yeast SF3b1through a UHM-ULM interaction Talkish J, Igel H, ..., Ares M 10.1261/rna.070649.119
28062854 SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity ofbranchsite selection in yeast Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA 10.1093/nar/gkw1349