Product Description: single-stranded DNA-binding protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0042645 | mitochondrial nucleoid |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0009295 | nucleoid |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0006264 | mitochondrial DNA replication |
| Process | GO:0051096 | positive regulation of helicase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.27 | 0.83 |
| Schizont | expressed | 0.27 | 0.82 |
| Gametocyte | not expressed | 0.04 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0508800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.151 | MFS: -2.837 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1108400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1108400)
Modification: Disrupted | RMgm-2965
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I415
"Best" AlphaFill ligand hit: TMP (thymidine-5'-phosphate, Local RMSD=0.31) with 3UDG (Global RMSD=1.46)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101436
Most Similar Human Ortholog: E7EUY5
TM-align score: 0.75 | RMSD: 3.34
Seq Identity: 0.32 | Length: 126 / 284
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000106028 | single stranded DNA binding protein 1 |
| ENSG00000262771 | single stranded DNA binding protein 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 226
Non-coding: 205 | Synonymous: 10 | Nonsynonymous: 9 | Stop Codon: 2
Protein Length: 284 | Molecular Weight (kDa): 33.86
UniProt ID(s): Q8I415
PDB ID(s): 3ULP
Isoelectric Point: 8.4
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000424 | Primosome PriB/single-strand DNA-binding |
| InterPro | IPR011344 | Single-stranded DNA-binding protein |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| PFam | PF00436 | Primosome PriB/single-strand DNA-binding |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20571080 | Single-stranded DNA binding protein from human malarial parasite Plasmodiumfalciparum is encoded in the nucleus and targeted to the apicoplast. | Prusty D, Dar A, ..., Dhar SK | 10.1093/nar/gkq565 |
| 22543238 | Plasmodium falciparum SSB tetramer binds single-stranded DNA only in a fullywrapped mode. | Antony E, Kozlov AG, Nguyen B, Lohman TM | 10.1016/j.jmb.2012.04.022 |
| 24267922 | Plasmodium falciparum single-stranded DNA-binding protein (PfSSB) interacts withPfPrex helicase and modulates its activity. | Bhowmick K, Dhar SK | 10.1111/1574-6968.12343 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |