PF3D7_0509000 (alphaSNAP)

Genome location: Pf3D7_05_v3:376,542..379,117(-)

Genome classification: Core

Function and Localization

Product Description: alpha-soluble NSF attachment protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0031201	SNARE complex
Component	GO:0005783	endoplasmic reticulum
Component	GO:0005774	vacuolar membrane
Function	GO:0005483	soluble NSF attachment protein activity
Function	GO:0019905	syntaxin binding
Process	GO:0035494	SNARE complex disassembly
Process	GO:0006886	intracellular protein transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.05	0.98
Trophozoite	expressed	0.21	0.87
Schizont	expressed	0.12	0.91
Gametocyte	expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0509000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.363 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1108600)

Relative Growth Rate: 0.33 ± 0.61
Confidence: 2.37

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I0X0

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101315

Most Similar Human Ortholog: M0R058

TM-align score: 0.92 | RMSD: 1.30

Seq Identity: 0.33 | Length: 136 / 298

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000105402	NSF attachment protein alpha
ENSG00000125814	NSF attachment protein beta

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	9	7	20
disruptive	1	8	4	32
missense	0	8	4	28

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	28	15	16
disruptive	4	26	29	72
missense	0	17	24	58

PlasmoDB Total SNPs: 119

Non-coding: 112 | Synonymous: 6 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 298 | Molecular Weight (kDa): 34.646

UniProt ID(s): Q8I0X0

PDB ID(s): None

Isoelectric Point: 5.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000744	NSF attachment protein
InterPro	IPR011990	Tetratricopeptide-like helical domain superfamily
PFam	PF14938	N/A
Superfamily	SSF48452	Tetratricopeptide-like helical domain superfamily