PF3D7_0509200 (LRR2)

Genome location: Pf3D7_05_v3:386,956..388,505(+)

Genome classification: Core

Function and Localization

Product Description: leucine-rich repeat protein

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0003774	cytoskeletal motor activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.12	0.93
Schizont	not expressed	0.04	0.97
Gametocyte	possibly expressed	0.61	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0509200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.703 | MFS: -2.94 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1108800)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1108800)

Modification: Disrupted | RMgm-2967

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I412

"Best" AlphaFill ligand hit: 15P (peg 1500, Local RMSD=1.25) with 6F4J (Global RMSD=5.58)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102611

Most Similar Human Ortholog: Q8NEP3

TM-align score: 0.70 | RMSD: 2.11

Seq Identity: 0.32 | Length: 253 / 338

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000154099	dynein axonemal assembly factor 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	12	13	22
disruptive	6	30	19	41
missense	6	26	18	39

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	31	19	16
disruptive	14	72	21	63
missense	9	58	17	50

PlasmoDB Total SNPs: 80

Non-coding: 59 | Synonymous: 17 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 338 | Molecular Weight (kDa): 40.092

UniProt ID(s): Q2TFW9, Q8I412

PDB ID(s): None

Isoelectric Point: 8.63

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR027734	Dynein assembly factor 1, axonemal
PFam	N/A	N/A
Superfamily	SSF52058	N/A