PF3D7_0509500

Genome location: Pf3D7_05_v3:401,124..402,771(+)

Genome classification: Core

Function and Localization

Product Description: ERCC4 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0048476	Holliday junction resolvase complex
Component	GO:0005634	nucleus
Function	GO:0048257	3'-flap endonuclease activity
Process	GO:0000727	double-strand break repair via break-induced replication
Process	GO:0031573	mitotic intra-S DNA damage checkpoint signaling
Process	GO:0000712	resolution of meiotic recombination intermediates

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.24	0.88
Trophozoite	not expressed	0.53	0.70
Schizont	not expressed	0.22	0.86
Gametocyte	possibly expressed	0.36	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0509500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -3.048 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1109100)

Relative Growth Rate: 0.24 ± 0.13
Confidence: 5.53

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1109100)

Modification: Disrupted | RMgm-2970

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I409

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156408

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	13	7	20
disruptive	19	40	27	62
missense	8	33	23	45

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	24	13	29
disruptive	27	114	40	98
missense	12	76	35	71

PlasmoDB Total SNPs: 79

Non-coding: 33 | Synonymous: 27 | Nonsynonymous: 19 | Stop Codon: 0

Protein Information

Protein Length: 444 | Molecular Weight (kDa): 52.317

UniProt ID(s): Q8I409

PDB ID(s): None

Isoelectric Point: 8.88

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006166	ERCC4 domain
InterPro	IPR033310	Mms4/EME1/EME2
PFam	PF02732	ERCC4 domain
Superfamily	N/A	N/A