Product Description: asparagine--tRNA ligase
SignalP Peptide: MRIYFITLIYFFTLLFLLTKC
# Transmembrane Domains: 1
EC Numbers: 6.1.1.22 (Asparagine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Function | GO:0004816 | asparagine-tRNA ligase activity |
| Process | GO:0006421 | asparaginyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.23 | 0.89 |
| Trophozoite | expressed | 0.63 | 0.64 |
| Schizont | expressed | 0.13 | 0.91 |
| Gametocyte | expressed | 0.46 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0509600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.018 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1109200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I408
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.33) with 3REX (Global RMSD=2.94)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.22 | asparagine-tRNA ligase | 3D6NH4+NO3-CNS-LCM01LCM02CH3COO-NSC12156NSC35467NSC114691NSC363624variolin B... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101288
Most Similar Human Ortholog: Q96I59
TM-align score: 0.90 | RMSD: 2.07
Seq Identity: 0.37 | Length: 449 / 722
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000137513 | asparaginyl-tRNA synthetase 2, mitochondrial |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 31 | 12 | 41 |
| disruptive | 18 | 85 | 52 | 96 |
| missense | 10 | 59 | 38 | 72 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 66 | 33 | 42 |
| disruptive | 41 | 177 | 69 | 143 |
| missense | 17 | 127 | 52 | 98 |
PlasmoDB Total SNPs: 147
Non-coding: 93 | Synonymous: 36 | Nonsynonymous: 18 | Stop Codon: 0
Protein Length: 722 | Molecular Weight (kDa): 85.249
UniProt ID(s): Q8I408
PDB ID(s): None
Isoelectric Point: 9.18
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002312 | Aspartyl/Asparaginyl-tRNA synthetase, class IIb |
| InterPro | IPR004364 | Aminoacyl-tRNA synthetase, class II (D/K/N) |
| InterPro | IPR004365 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| InterPro | IPR004522 | Asparagine-tRNA ligase |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| PFam | PF00152 | Aminoacyl-tRNA synthetase, class II (D/K/N) |
| PFam | PF01336 | OB-fold nucleic acid binding domain, AA-tRNA synthetase-type |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF55681 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 24196969 | Aminoacylation of Plasmodium falciparum tRNA(Asn) and insights in the synthesisof asparagine repeats. | Filisetti D, Theobald-Dietrich A, ..., Frugier M | 10.1074/jbc.M113.522896 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 37546892 | Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNAsynthetase | Xie SC, Wang Y, ..., Tilley L | 10.21203/rs.3.rs-3198291/v1 |
| 38297033 | Reaction hijacking inhibition of Plasmodium falciparum asparagine tRNAsynthetase | Xie SC, Wang Y, ..., Tilley L | 10.1038/s41467-024-45224-z |