Product Description: phosphatidylinositol 4-kinase beta
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.67 (1-phosphatidylinositol 4-kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0016020 | membrane |
| Function | GO:0004430 | 1-phosphatidylinositol 4-kinase activity |
| Function | GO:0052742 | phosphatidylinositol kinase activity |
| Process | GO:0006661 | phosphatidylinositol biosynthetic process |
| Process | GO:0046488 | phosphatidylinositol metabolic process |
| Process | GO:0046854 | phosphatidylinositol phosphate biosynthetic process |
| Process | GO:0048015 | phosphatidylinositol-mediated signaling |
| Process | GO:0042493 | response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.04 | 0.54 |
| Trophozoite | expressed | 0.97 | 0.50 |
| Schizont | expressed | 1.21 | 0.42 |
| Gametocyte | expressed | 0.96 | 0.50 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0509800
Resistome Missense Mutations: S743F, E1316D, E1316D, S1320L, I1354M, Y1356C, Y1356N, L1418F, L1418F, H1484Y
Resistome Compounds with Missense Mutations: BRD73842, KAI407, MMV010545, Sapanisertib
Resistome # Samples with Disruptive Mutations: 22 (16 missense, 16 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.134 | MFS: -3.011 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1109400)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1109400)
Modification: Mutated | RMgm-969
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I406
"Best" AlphaFill ligand hit: GSP (5'-guanosine-diphosphate-monothiophosphate, Local RMSD=0.12) with 4D0L (Global RMSD=1.66)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.67 | 1-phosphatidylinositol 4-kinase | ATPADPAMPGTPGDPCTPUTPKClNEMSDSGMPCDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9UBF8 | Phosphatidylinositol 4-kinase beta | OrthoDB, OrthoMCL BLAST | US10112926, Example 1US10112926, Example 2US10112926, Example 3US10112926, Example 4... | |
| Q5CVD3 | Phosphatidylinositol 4-kinase | OrthoDB, OrthoMCL, OrthoMCL BLAST | WO2022079616, Example 1.01WO2022079616, Example 1.02WO2022079616, Example 1.03WO2022079616, Example 1.04... | |
| P42356 | Phosphatidylinositol 4-kinase alpha | OrthoDB | US11091472, Compound S-145US11091472, Compound S-147US11091472, Compound S-148US11091472, Compound S-169... |
Ortholog Group (OrthoMCL): OG6_101543
Most Similar Human Ortholog: H0YCW3
TM-align score: 0.87 | RMSD: 1.72
Seq Identity: 0.42 | Length: 170 / 1559
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000143393 | phosphatidylinositol 4-kinase beta |
Protein Length: 1559 | Molecular Weight (kDa): 182.664
UniProt ID(s): Q8I406
PDB ID(s): None
Isoelectric Point: 5.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000403 | Phosphatidylinositol 3-/4-kinase, catalytic domain |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR015433 | Phosphatidylinositol kinase |
| InterPro | IPR016024 | Armadillo-type fold |
| InterPro | IPR018936 | Phosphatidylinositol 3/4-kinase, conserved site |
| PFam | PF00454 | Phosphatidylinositol 3-/4-kinase, catalytic domain |
| Superfamily | SSF48371 | Armadillo-type fold |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 73 | 34 | 81 |
| disruptive | 92 | 191 | 73 | 240 |
| missense | 42 | 114 | 49 | 167 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 136 | 55 | 89 |
| disruptive | 161 | 364 | 139 | 342 |
| missense | 69 | 227 | 102 | 213 |
PlasmoDB Total SNPs: 338
Non-coding: 152 | Synonymous: 79 | Nonsynonymous: 107 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 20381540 | Complementation of Saccharomyces cerevisiaepik1ts by a phosphatidylinositol4-kinase from Plasmodium falciparum. | Kruger T, Sanchez CP, Lanzer M | 10.1016/j.molbiopara.2010.03.020 |
| 24284631 | Targeting Plasmodium PI(4)K to eliminate malaria. | McNamara CW, Lee MC, ..., Winzeler EA | 10.1038/nature12782 |
| 28446690 | Antimalarial efficacy of M MV390048, an inhibitor of Plasmodiumphosphatidylinositol 4-kinase | Paquet T, Le Manach C, ..., Chibale K | 10.1126/scitranslmed.aad9735 |
| 32543181 | Heterologous Expression, Purification, and Functional Analysis of the Plasmodiumfalciparum Phosphatidylinositol 4-Kinase IIIbeta. | Sternberg AR, Roepe PD | 10.1021/acs.biochem.0c00259 |