PF3D7_0509900

Genome location: Pf3D7_05_v3:415,972..417,680(+)

Genome classification: Core

Function and Localization

Product Description: NSE4 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	not expressed	0.14	0.91
Schizont	not expressed	0.06	0.96
Gametocyte	not expressed	0.04	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0509900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.177 | MFS: -3.412 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1109500)

Relative Growth Rate: 0.21 ± 0.14
Confidence: 5.27

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I405

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.64) with 3A4L (Global RMSD=25.62)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156409

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	11	9	14
disruptive	3	16	16	30
missense	3	14	15	24

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	22	14	21
disruptive	11	42	34	92
missense	8	31	26	53

PlasmoDB Total SNPs: 72

Non-coding: 62 | Synonymous: 6 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 314 | Molecular Weight (kDa): 37.175

UniProt ID(s): Q8I405

PDB ID(s): None

Isoelectric Point: 9.01

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR014854	Non-structural maintenance of chromosome element 4, C-terminal
PFam	PF08743	Non-structural maintenance of chromosome element 4, C-terminal
Superfamily	N/A	N/A