PF3D7_0510200 (CYP87)

Genome location: Pf3D7_05_v3:436,017..439,924(+)

Genome classification: Core

Function and Localization

Product Description: peptidyl-prolyl cis-trans isomerase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.2.1.8 (Peptidylprolyl isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016018	cyclosporin A binding
Function	GO:0003755	peptidyl-prolyl cis-trans isomerase activity
Process	GO:0006457	protein folding
Process	GO:0000413	protein peptidyl-prolyl isomerization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.49	0.77
Trophozoite	expressed	0.63	0.65
Schizont	expressed	0.43	0.74
Gametocyte	expressed	0.67	0.63

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0510200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.206 | MFS: -2.598 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1109800)

Relative Growth Rate: 0.52 ± 0.17
Confidence: 4.92

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1109800)

Modification: Disrupted | RMgm-2977

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I402

"Best" AlphaFill ligand hit: LSA (saccharin, Local RMSD=0.07) with 4N1O (Global RMSD=2.27)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.2.1.8	peptidylprolyl isomerase	ATPNEMSDSPCMBFK506QAEGPKAla-ProjugloneSer-ProGPI-1046RNase T1Rapamycin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102016

Most Similar Human Ortholog: D6R9Q1

TM-align score: 0.93 | RMSD: 1.62

Seq Identity: 0.35 | Length: 238 / 747

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000113593	peptidylprolyl isomerase domain and WD repeat containing 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	26	18	39
disruptive	10	59	23	77
missense	5	48	18	58

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	67	19	33
disruptive	30	114	72	187
missense	14	83	50	106

PlasmoDB Total SNPs: 166

Non-coding: 113 | Synonymous: 39 | Nonsynonymous: 14 | Stop Codon: 0

Protein Information

Protein Length: 747 | Molecular Weight (kDa): 87.04

UniProt ID(s): Q8I402

PDB ID(s): 2FU0

Isoelectric Point: 7.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR002130	Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
InterPro	IPR020892	Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site
InterPro	IPR029000	Cyclophilin-like domain superfamily
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	PF00160	Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
Superfamily	SSF50891	Cyclophilin-like domain superfamily
Superfamily	SSF50978	WD40-repeat-containing domain superfamily