Product Description: topoisomerase I
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.99.1.2 (Transferred entry: 5.6.2.1)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005730 | nucleolus |
| Component | GO:0005634 | nucleus |
| Component | GO:0031298 | replication fork protection complex |
| Function | GO:0003917 | DNA topoisomerase type I (single strand cut, ATP-independent) activity |
| Process | GO:0006260 | DNA replication |
| Process | GO:0006265 | DNA topological change |
| Process | GO:0006338 | chromatin remodeling |
| Process | GO:0007059 | chromosome segregation |
| Process | GO:0042493 | response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.46 | 0.79 |
| Trophozoite | expressed | 1.27 | 0.39 |
| Schizont | expressed | 0.62 | 0.67 |
| Gametocyte | expressed | 0.75 | 0.61 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0510500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.151 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3Z9
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 5.99.1.2 | DNA topoisomerase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P11387 | DNA topoisomerase 1 | OMA, OrthoDB | CHEMBL4064887CHEMBL4065223CHEMBL4076393CHEMBL4064127... | |
| Q04750 | DNA topoisomerase 1 | Mus musculus | OMA, OrthoDB | CHEMBL289665SN-38CHEMBL453303CHEMBL507686... |
| P04786 | DNA topoisomerase 1 | OMA, OrthoDB, OrthoMCL | combretastatins A-1combretastatins A-1 | |
| Q9WUL0 | DNA topoisomerase 1 | Rattus norvegicus | OrthoDB | acs.jmedchem.1c00409_ST.600acs.jmedchem.1c00409_ST.600 |
| E9B7X3 | DNA topoisomerase type IB small subunit | OrthoMCL BLAST | Camptothecin (CPT)Camptothecin (CPT) | |
| Q95Z89 | Topoisomerase I subunit B | Leishmania major | OrthoMCL BLAST | CHEMBL2153502Camptothecin (CT) |
| Q16611 | Bcl-2 homologous antagonist/killer | OrthoMCL BLAST | Apogossypol derivative, 8kCHEMBL5203189CHEMBL5182721CHEMBL605114... | |
| Q2HPH0 | Topoisomerase I | OrthoMCL BLAST | CHEMBL4176420CHEMBL4159820 |
Ortholog Group (OrthoMCL): OG6_101409
Most Similar Human Ortholog: E5RFS0
TM-align score: 0.95 | RMSD: 1.35
Seq Identity: 0.38 | Length: 221 / 839
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000184428 | DNA topoisomerase I mitochondrial |
| ENSG00000198900 | DNA topoisomerase I |
Protein Length: 839 | Molecular Weight (kDa): 98.11
PDB ID(s): None
Isoelectric Point: 9.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001631 | DNA topoisomerase I |
| InterPro | IPR008336 | DNA topoisomerase I, DNA binding, eukaryotic-type |
| InterPro | IPR011010 | DNA breaking-rejoining enzyme, catalytic core |
| InterPro | IPR013499 | DNA topoisomerase I, eukaryotic-type |
| InterPro | IPR013500 | DNA topoisomerase I, catalytic core, eukaryotic-type |
| InterPro | IPR018521 | DNA topoisomerase I, active site |
| InterPro | IPR025834 | Topoisomerase I C-terminal domain |
| InterPro | IPR036202 | DNA topoisomerase I, DNA binding, eukaryotic-type, N-terminal domain superfamily |
| PFam | PF01028 | DNA topoisomerase I, catalytic core, eukaryotic-type |
| PFam | PF02919 | DNA topoisomerase I, DNA binding, eukaryotic-type |
| PFam | PF14370 | Topoisomerase I C-terminal domain |
| Superfamily | SSF56349 | DNA breaking-rejoining enzyme, catalytic core |
| Superfamily | SSF56741 | DNA topoisomerase I, DNA binding, eukaryotic-type, N-terminal domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 24 | 27 | 35 |
| disruptive | 9 | 44 | 30 | 45 |
| missense | 9 | 43 | 28 | 39 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 73 | 16 | 31 |
| disruptive | 22 | 105 | 46 | 102 |
| missense | 16 | 80 | 37 | 74 |
PlasmoDB Total SNPs: 53
Non-coding: 22 | Synonymous: 24 | Nonsynonymous: 7 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 22446744 | Mapping the genome of Plasmodium falciparum on the drug-like chemical spacereveals novel anti-malarial targets and potential drug leads. | Jensen K, Plichta D, Panagiotou G, Kouskoumvekaki I | 10.1039/c2mb00008c |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24651068 | Theoretical and experimental studies of new modified isoflavonoids as potentialinhibitors of topoisomerase I from Plasmodium falciparum. | Cortopassi WA, Penna-Coutinho J, ..., Krettli AU | 10.1371/journal.pone.0091191 |
| 36983048 | StaR Is a Positive Regulator of Topoisomerase I Activity Involved in SupercoilingMaintenance in Streptococcus pneumoniae | de Vasconcelos Junior AA, Tirado-Velez JM, ..., de la Campa AG | 10.3390/ijms24065973 |