Product Description: RNA pseudouridylate synthase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.4.99.12 (tRNA pseudouridine(38-40) synthase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0005634 | nucleus |
Function | GO:0003729 | mRNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | not expressed | N/A | N/A |
Ring | expressed | 3.88 | 0.05 |
Trophozoite | expressed | 3.65 | 0.01 |
Schizont | expressed | 3.02 | 0.09 |
Gametocyte | not expressed | 2.86 | 0.07 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0511500
Resistome Missense Mutations: K2762E
Resistome Compounds with Missense Mutations: MMV688533
Resistome # Samples with Disruptive Mutations: 2 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.927 | #Insertions: 26
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1111000)
Modification: Disrupted | RMgm-2983
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
5.4.99.12 | tRNA pseudouridine38-40 synthase | tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 112 | 392 | 222 | 527 |
disruptive | 447 | 1207 | 676 | 1570 |
missense | 209 | 822 | 510 | 1268 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 199 | 698 | 368 | 599 |
disruptive | 794 | 2355 | 1115 | 2400 |
missense | 348 | 1634 | 864 | 1670 |
PlasmoDB Total SNPs: 1186
Non-coding: 72 | Synonymous: 685 | Nonsynonymous: 429 | Stop Codon: 0
Protein Length: 10061 | Molecular Weight (kDa): 1187.585
UniProt ID(s): Q8I3Z1
PDB ID(s): None
Isoelectric Point: 7.53
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR002083 | MATH/TRAF domain |
InterPro | IPR006145 | Pseudouridine synthase, RsuA/RluA |
InterPro | IPR008974 | TRAF-like |
InterPro | IPR016024 | Armadillo-type fold |
InterPro | IPR020103 | Pseudouridine synthase, catalytic domain superfamily |
PFam | N/A | N/A |
Superfamily | SSF48371 | Armadillo-type fold |
Superfamily | SSF49599 | TRAF-like |
Superfamily | SSF55120 | Pseudouridine synthase, catalytic domain superfamily |
PMID | Title | Authors | DOI/Link |
---|---|---|---|
12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
15477199 | Myosin-like sequences in the malaria parasite Plasmodium falciparum bind humanerythrocyte membrane protein 4.1. | Lanzillotti R, Coetzer TL | https://pubmed.ncbi.nlm.nih.gov/15477199/ |
16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |