About Gene List

PF3D7_0511500

Genome location: Pf3D7_05_v3:484,937..517,423(+)

Genome classification: Core

Function and Localization

Product Description: RNA pseudouridylate synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.4.99.12 (tRNA pseudouridine(38-40) synthase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus
Function GO:0003729 mRNA binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 3.88 0.05
Trophozoite expressed 3.65 0.01
Schizont expressed 3.02 0.09
Gametocyte not expressed 2.86 0.07

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0511500

Resistome Missense Mutations: K2762E

Resistome Compounds with Missense Mutations: MMV688533

Resistome # Samples with Disruptive Mutations: 2 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.927 | #Insertions: 26

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1111000)

Modification: Disrupted | RMgm-2983

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
5.4.99.12 tRNA pseudouridine38-40 synthase tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533345

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 112 392 222 527
disruptive 447 1207 676 1570
missense 209 822 510 1268

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 199 698 368 599
disruptive 794 2355 1115 2400
missense 348 1634 864 1670

PlasmoDB Total SNPs: 1186

Non-coding: 72 | Synonymous: 685 | Nonsynonymous: 429 | Stop Codon: 0

Protein Information

Protein Length: 10061 | Molecular Weight (kDa): 1187.585

UniProt ID(s): Q8I3Z1

PDB ID(s): None

Isoelectric Point: 7.53

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002083 MATH/TRAF domain
InterPro IPR006145 Pseudouridine synthase, RsuA/RluA
InterPro IPR008974 TRAF-like
InterPro IPR016024 Armadillo-type fold
InterPro IPR020103 Pseudouridine synthase, catalytic domain superfamily
PFam N/A N/A
Superfamily SSF48371 Armadillo-type fold
Superfamily SSF49599 TRAF-like
Superfamily SSF55120 Pseudouridine synthase, catalytic domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
15477199 Myosin-like sequences in the malaria parasite Plasmodium falciparum bind humanerythrocyte membrane protein 4.1. Lanzillotti R, Coetzer TL https://pubmed.ncbi.nlm.nih.gov/15477199/
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
27381095 The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. Bunnik EM, Batugedara G, ..., Le Roch KG 10.1186/s13059-016-1014-0