Product Description: glutathione synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.2.3 (Glutathione synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0043295 | glutathione binding |
| Function | GO:0004363 | glutathione synthase activity |
| Process | GO:0006750 | glutathione biosynthetic process |
| Process | GO:0006979 | response to oxidative stress |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.30 | 0.83 |
| Schizont | expressed | 0.04 | 0.98 |
| Gametocyte | expressed | 0.25 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0512200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.133 | MFS: -2.816 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1111800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1111800)
Modification: Disrupted | RMgm-2984
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3Y4
"Best" AlphaFill ligand hit: GSH (glutathione, Local RMSD=0.20) with 2WYO (Global RMSD=6.47)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.3 | glutathione synthase | ATPADPAMPNEMPCMBGSSGNADPHc-junphosphateadenosinemenadioneL-cystine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101596
Most Similar Human Ortholog: P48637
TM-align score: 0.94 | RMSD: 2.10
Seq Identity: 0.27 | Length: 473 / 655
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100983 | glutathione synthetase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 35 | 20 | 35 |
| disruptive | 28 | 57 | 39 | 93 |
| missense | 24 | 51 | 32 | 75 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 19 | 56 | 23 | 41 |
| disruptive | 44 | 130 | 71 | 124 |
| missense | 37 | 102 | 59 | 97 |
PlasmoDB Total SNPs: 155
Non-coding: 94 | Synonymous: 29 | Nonsynonymous: 32 | Stop Codon: 0
Protein Length: 655 | Molecular Weight (kDa): 77.481
PDB ID(s): None
Isoelectric Point: 7.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004887 | Glutathione synthase, substrate-binding domain |
| InterPro | IPR005615 | Glutathione synthase |
| InterPro | IPR016185 | Pre-ATP-grasp domain superfamily |
| PFam | PF03199 | Glutathione synthase, substrate-binding domain |
| PFam | PF03917 | Glutathione synthase |
| Superfamily | SSF52440 | Pre-ATP-grasp domain superfamily |
| Superfamily | SSF56059 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |