Product Description: ras-related protein Rab-1B
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.2 (Small monomeric GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005801 | cis-Golgi network |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0005793 | endoplasmic reticulum-Golgi intermediate compartment |
| Component | GO:0020020 | food vacuole |
| Function | GO:0005515 | protein binding |
| Process | GO:0015031 | protein transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.54 | 0.68 |
| Schizont | expressed | 0.45 | 0.71 |
| Gametocyte | expressed | 0.68 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0512600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.249 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K8H7
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.10) with 3LAW (Global RMSD=2.66)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.2 | small monomeric GTPase | GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P62834 | Ras-related protein Rap-1A | Homo sapiens | OrthoDB | CHEMBL159171 |
| P01116 | GTPase KRas | Homo sapiens | OrthoDB | US11213515, TABLE 7.22US11213515, TABLE 7.62US11213515, TABLE 7.106US11213515, TABLE 7.190... |
| P01112 | GTPase HRas | Homo sapiens | OrthoDB | CHEMBL324827CHEMBL326352CHEMBL431440CHEMBL324345... |
| P01111 | GTPase NRas | Homo sapiens | OrthoDB | NCGC 00371479NCGC 00371480NCGC 00371484NCGC 00371485... |
| P01120 | Ras-like protein 2 | OrthoDB | Arteminolide C'Arteminolide C | |
| Q9BSW2 | EF-hand calcium-binding domain-containing protein 4B | OrthoMCL BLAST | US8921364, 1US8921364, 2US8921364, 4US8921364, 7... |
Ortholog Group (OrthoMCL): OG6_101123
Most Similar Human Ortholog: P62820
TM-align score: 0.86 | RMSD: 1.30
Seq Identity: 0.72 | Length: 176 / 200
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000138069 | RAB1A, member RAS oncogene family |
| ENSG00000174903 | RAB1B, member RAS oncogene family |
Protein Length: 200 | Molecular Weight (kDa): 22.886
UniProt ID(s): A0A5K1K8H7, Q9BHN1
PDB ID(s): None
Isoelectric Point: 6.48
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001806 | Small GTPase |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00071 | Small GTPase |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 12 | 5 | 11 |
| disruptive | 1 | 1 | 4 | 6 |
| missense | 1 | 1 | 4 | 6 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 16 | 10 | 11 |
| disruptive | 1 | 10 | 10 | 29 |
| missense | 1 | 7 | 9 | 20 |
PlasmoDB Total SNPs: 184
Non-coding: 174 | Synonymous: 10 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 12657463 | The Plasmodium falciparum family of Rab GTPases. | Quevillon E, Spielmann T, ..., Langsley G | 10.1016/s0378-1119(03)00381-0 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 33604307 | Rab5b-Associated Arf1 GTPase Regulates Export of N-Myristoylated Adenylate Kinase2 From the Endoplasmic Reticulum in Plasmodium falciparum. | Taku I, Hirai T, ..., Saito-Nakano Y | 10.3389/fcimb.2020.610200 |
| 37076474 | Dephosphocholination by Legionella effector Lem3 functions through remodelling ofthe switch II region of Rab1b | Kaspers MS, Pogenberg V, ..., Itzen A | 10.1038/s41467-023-37621-7 |
| 37941035 | Transcriptomic and metabolomic analyses reveal the essential nature of Rab1B inToxoplasma gondii | He K, Wang Q, ..., Long S | 10.1186/s13071-023-06030-6 |
| 37548452 | Functional screening reveals Toxoplasma prenylated proteins required forendocytic trafficking and rhoptry protein sorting | Wang QQ, Sun M, ..., Long S | 10.1128/mbio.01309-23 |
| 38407123 | Deciphering protein prenylation in endocytic trafficking in Toxoplasma gondii | Carruthers VB, Dou Z | 10.1128/mbio.00283-24 |