Product Description: orotate phosphoribosyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.4.2.10 (Orotate phosphoribosyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004588 | orotate phosphoribosyltransferase activity |
| Function | GO:0005515 | protein binding |
| Function | GO:0046983 | protein dimerization activity |
| Process | GO:0006207 | 'de novo' pyrimidine nucleobase biosynthetic process |
| Process | GO:0006221 | pyrimidine nucleotide biosynthetic process |
| Process | GO:0046132 | pyrimidine ribonucleoside biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.28 | 0.86 |
| Trophozoite | expressed | 0.70 | 0.64 |
| Schizont | expressed | 0.06 | 0.97 |
| Gametocyte | expressed | 0.33 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0512700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.214 | MFS: -3.229 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0507400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1112400)
Modification: Disrupted | RMgm-1267
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3Y0
"Best" AlphaFill ligand hit: OMP (orotidine-5'-monophosphate, Local RMSD=0.45) with 2PRZ (Global RMSD=2.17)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.2.10 | orotate phosphoribosyltransferase | ATPADPUDPAMPUTPNEMUMPGMPIMPEDTAPCMBdUMP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8I3Y0 | Orotate phosphoribosyltransferase | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | OPRT inhibitor, 1 OPRT inhibitor, 2OPRT inhibitor, 3OPRT inhibitor, 4... |
Ortholog Group (OrthoMCL): OG6_100519
Most Similar Human Ortholog: F2Z303
TM-align score: 0.78 | RMSD: 1.86
Seq Identity: 0.20 | Length: 65 / 281
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000114491 | uridine monophosphate synthetase |
Protein Length: 281 | Molecular Weight (kDa): 33.006
PDB ID(s): None
Isoelectric Point: 7.19
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000836 | Phosphoribosyltransferase domain |
| InterPro | IPR023031 | Orotate phosphoribosyltransferase |
| InterPro | IPR029057 | Phosphoribosyltransferase-like |
| PFam | PF00156 | Phosphoribosyltransferase domain |
| Superfamily | SSF53271 | Phosphoribosyltransferase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 6 | 11 | 11 |
| disruptive | 9 | 21 | 9 | 31 |
| missense | 3 | 16 | 9 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 25 | 12 | 19 |
| disruptive | 14 | 46 | 19 | 57 |
| missense | 4 | 32 | 17 | 44 |
PlasmoDB Total SNPs: 104
Non-coding: 70 | Synonymous: 25 | Nonsynonymous: 8 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 15571277 | Cloning and expression of malarial pyrimidine enzymes. | Christopherson RI, Cinquin O, ..., Menz RI | 10.1081/NCN-200027678 |
| 15683248 | A novel enzyme complex of orotate phosphoribosyltransferase and orotidine5'-monophosphate decarboxylase in human malaria parasite Plasmodium falciparum:physical association, kinetics, and inhibition characterization. | Krungkrai SR, DelFraino BJ, ..., Krungkrai J | 10.1021/bi048439h |
| 19800871 | Kinetic benefits and thermal stability of orotate phosphoribosyltransferase andorotidine 5'-monophosphate decarboxylase enzyme complex in human malaria parasitePlasmodium falciparum. | Kanchanaphum P, Krungkrai J | 10.1016/j.bbrc.2009.09.128 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24158442 | Transition state analogues of Plasmodium falciparum and human orotatephosphoribosyltransferases. | Zhang Y, Evans GB, ..., Schramm VL | 10.1074/jbc.M113.521955 |
| 24507637 | A unique insertion of low complexity amino acid sequence underliesprotein-protein interaction in human malaria parasite orotatephosphoribosyltransferase and orotidine 5'-monophosphate decarboxylase. | Imprasittichail W, Roytrakul S, Krungkrai SR, Krungkrail J | 10.1016/S1995-7645(14)60018-3 |
| 25945715 | Structure of Plasmodium falciparum orotate phosphoribosyltransferase withautologous inhibitory protein-protein interactions. | Kumar S, Krishnamoorthy K, Mudeppa DG, Rathod PK | 10.1107/S2053230X1500549X |