Product Description: tubulin--tyrosine ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.3.2.25 (Tubulin--tyrosine ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005929 | cilium |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0015631 | tubulin binding |
| Function | GO:0070740 | tubulin-glutamic acid ligase activity |
| Process | GO:0000226 | microtubule cytoskeleton organization |
| Process | GO:0018095 | protein polyglutamylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | possibly expressed | 0.04 | 0.97 |
| Schizont | expressed | 0.12 | 0.92 |
| Gametocyte | expressed | 0.50 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0514000
Resistome Missense Mutations: R18K
Resistome Compounds with Missense Mutations: BI-2536
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.925 | MFS: -2.672 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1113700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1113700)
Modification: Disrupted | RMgm-2997
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4D8
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.47) with 6VZU (Global RMSD=3.09)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.25 | tubulin-tyrosine ligase | ADPAMPNEML-PheL-TyrL-DopatyraminestathminphosphateL-Tyr-GlyL-Tyr-L-GluL-Ala-L-Tyr... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104969
Most Similar Human Ortholog: A0A2R8Y6A5
TM-align score: 0.91 | RMSD: 1.55
Seq Identity: 0.41 | Length: 356 / 420
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000131044 | tubulin tyrosine ligase like 9 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 18 | 7 | 30 |
| disruptive | 2 | 6 | 11 | 29 |
| missense | 1 | 6 | 11 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 37 | 18 | 29 |
| disruptive | 7 | 41 | 29 | 67 |
| missense | 1 | 30 | 26 | 47 |
PlasmoDB Total SNPs: 122
Non-coding: 102 | Synonymous: 16 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 420 | Molecular Weight (kDa): 50.451
UniProt ID(s): C0H4D8
PDB ID(s): None
Isoelectric Point: 8.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004344 | Tubulin-tyrosine ligase/Tubulin polyglutamylase |
| InterPro | IPR027751 | Probable tubulin polyglutamylase TTLL9 |
| PFam | PF03133 | Tubulin-tyrosine ligase/Tubulin polyglutamylase |
| Superfamily | SSF56059 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |