Product Description: ATP-dependent DNA helicase UvrD
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0043138 | 3'-5' DNA helicase activity |
| Function | GO:0005515 | protein binding |
| Function | GO:0017116 | single-stranded DNA helicase activity |
| Process | GO:0006281 | DNA repair |
| Process | GO:0000725 | recombinational repair |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.07 | 0.96 |
| Trophozoite | expressed | 0.55 | 0.69 |
| Schizont | expressed | 0.54 | 0.68 |
| Gametocyte | expressed | 0.85 | 0.55 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0514100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.856 | #Insertions: 9
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1113800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1113800)
Modification: Disrupted | RMgm-2998
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3W6
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.15) with 2IS6 (Global RMSD=2.62)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8I3W6 | DNA helicase [35-727] | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | CiprofloxacinEthidium bromideDAUNORUBICINNETROPSIN... |
Protein Length: 1441 | Molecular Weight (kDa): 170.292
UniProt ID(s): A0A097PTD0, B8XWZ5, B9VPM5, Q8I3W6
PDB ID(s): None
Isoelectric Point: 8.17
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000212 | DNA helicase, UvrD/REP type |
| InterPro | IPR014017 | UvrD-like DNA helicase, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR034739 | UvrD/AddA helicase, N-terminal |
| PFam | PF00580 | UvrD/AddA helicase, N-terminal |
| PFam | PF13361 | UvrD-like DNA helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 57 | 50 | 83 |
| disruptive | 90 | 194 | 98 | 209 |
| missense | 46 | 144 | 82 | 165 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 116 | 54 | 97 |
| disruptive | 157 | 351 | 158 | 343 |
| missense | 84 | 254 | 123 | 235 |
PlasmoDB Total SNPs: 200
Non-coding: 23 | Synonymous: 99 | Nonsynonymous: 77 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23185322 | Plasmodium falciparum UvrD helicase translocates in 3' to 5' direction,colocalizes with MLH and modulates its activity through physical interaction. | Ahmad M, Ansari A, ..., Tuteja R | 10.1371/journal.pone.0049385 |
| 37839213 | UvrD-like helicase Hmi1 Has an ATP independent role in yeast mitochondrial DNAmaintenance | Sillamaa S, Piljukov VJ, ..., Sedman J | 10.1016/j.dnarep.2023.103582 |