PF3D7_0514300

Genome location: Pf3D7_05_v3:599,959..603,868(+)

Genome classification: Core

Function and Localization

Product Description: aspartate--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.1.1.12 (Aspartate--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Function	GO:0004815	aspartate-tRNA ligase activity
Process	GO:0006422	aspartyl-tRNA aminoacylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.13	0.94
Trophozoite	expressed	0.87	0.54
Schizont	expressed	0.32	0.81
Gametocyte	possibly expressed	0.65	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0514300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.148 | MFS: -2.8 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3W4

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.27) with 3NEM (Global RMSD=1.82)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.12	aspartate-tRNA ligase	KClNH4+tRNANO3-IO3-CNS-RNAiCaCl2NiCl2ClO3-K2HPO4CH3COO-...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532781

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	9	45	25	64
disruptive	53	138	72	192
missense	34	104	58	152

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	90	26	69
disruptive	95	298	127	334
missense	51	218	98	196

PlasmoDB Total SNPs: 236

Non-coding: 48 | Synonymous: 120 | Nonsynonymous: 68 | Stop Codon: 0

Protein Information

Protein Length: 1128 | Molecular Weight (kDa): 134.776

UniProt ID(s): Q8I3W4

PDB ID(s): None

Isoelectric Point: 9.21

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb
InterPro	IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)
InterPro	IPR012340	Nucleic acid-binding, OB-fold
PFam	PF00152	Aminoacyl-tRNA synthetase, class II (D/K/N)
Superfamily	SSF50249	Nucleic acid-binding, OB-fold
Superfamily	SSF55681

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895