PF3D7_0514600

Genome location: Pf3D7_05_v3:606,433..609,049(-)

Genome classification: Core

Function and Localization

Product Description: ribose-5-phosphate isomerase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.3.1.6 (Ribose-5-phosphate isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004751	ribose-5-phosphate isomerase activity
Process	GO:0009052	pentose-phosphate shunt, non-oxidative branch
Process	GO:0008615	pyridoxine biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.05	0.97
Trophozoite	expressed	0.14	0.91
Schizont	expressed	0.01	0.99
Gametocyte	expressed	0.07	0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0514600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.134 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1114200)

Relative Growth Rate: 0.15 ± 0.31
Confidence: 3.75

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3W2

"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.01) with 3IXQ (Global RMSD=2.23)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.3.1.6	ribose-5-phosphate isomerase	AMPNEMNAD+EDTAPCMB5'-AMP3'-AMP5'-UMP5'-GMP5'-CMP3'-CMP3'-UMP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101105

Most Similar Human Ortholog: P49247

TM-align score: 0.94 | RMSD: 1.20

Seq Identity: 0.36 | Length: 230 / 236

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000153574	ribose 5-phosphate isomerase A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	13	14	11
disruptive	1	10	2	15
missense	1	10	2	12

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	34	13	11
disruptive	5	24	14	65
missense	2	18	12	43

PlasmoDB Total SNPs: 211

Non-coding: 195 | Synonymous: 15 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 236 | Molecular Weight (kDa): 26.205

UniProt ID(s): Q8I3W2

PDB ID(s): 2F8M

Isoelectric Point: 7.19

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004788	Ribose 5-phosphate isomerase, type A
InterPro	IPR037171	NagB/RpiA transferase-like
PFam	PF06026	Ribose 5-phosphate isomerase, type A
Superfamily	SSF100950	NagB/RpiA transferase-like
Superfamily	SSF75445