PF3D7_0514800

Genome location: Pf3D7_05_v3:615,232..618,202(-)

Genome classification: Core

Function and Localization

Product Description: inositol polyphosphate multikinase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.-.- (Transferring phosphorus-containing groups.);2.7.1.151 (Inositol-polyphosphate multikinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0051765	inositol tetrakisphosphate kinase activity
Function	GO:0008440	inositol-1,4,5-trisphosphate 3-kinase activity
Function	GO:0016301	kinase activity
Process	GO:0032958	inositol phosphate biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.25	0.88
Trophozoite	not expressed	0.53	0.69
Schizont	possibly expressed	0.19	0.88
Gametocyte	not expressed	0.26	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0514800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.128 | MFS: -3.316 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1114400)

Modification: Disrupted | RMgm-3001

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3W0

"Best" AlphaFill ligand hit: LU2 (luteolin, Local RMSD=0.20) with 6M8A (Global RMSD=4.69)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.-.-	Ex: hamamelose kinase	ATPADPCoAUDPAMPGDPKClUMPCDPCTPGTPUTP...
2.7.1.151	inositol-polyphosphate multikinase	gossypolWortmanninRose bengalellagic acidabscisic acidchlorogenic acidaurintricarboxylic acidinositol(1,3,4)-trisphosphateinositol(3,5,6)-trisphosphate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_138035

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	28	20	42
disruptive	19	113	70	155
missense	15	97	64	139

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	63	38	58
disruptive	55	255	127	266
missense	40	206	111	203

PlasmoDB Total SNPs: 105

Non-coding: 17 | Synonymous: 53 | Nonsynonymous: 33 | Stop Codon: 2

Protein Information

Protein Length: 903 | Molecular Weight (kDa): 107.373

UniProt ID(s): Q8I3W0

PDB ID(s): None

Isoelectric Point: 8.29

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005522	Inositol polyphosphate kinase
PFam	PF03770	Inositol polyphosphate kinase
Superfamily	SSF56104	N/A