Product Description: pre-mRNA-splicing factor CWC2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000974 | Prp19 complex |
| Component | GO:0071006 | U2-type catalytic step 1 spliceosome |
| Component | GO:0071007 | U2-type catalytic step 2 spliceosome |
| Component | GO:0005634 | nucleus |
| Component | GO:0005681 | spliceosomal complex |
| Function | GO:0017070 | U6 snRNA binding |
| Function | GO:0003729 | mRNA binding |
| Function | GO:0036002 | pre-mRNA binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0000398 | mRNA splicing, via spliceosome |
| Process | GO:0045787 | positive regulation of cell cycle |
| Process | GO:0000387 | spliceosomal snRNP assembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.56 | 0.73 |
| Trophozoite | expressed | 0.93 | 0.50 |
| Schizont | possibly expressed | 0.46 | 0.71 |
| Gametocyte | possibly expressed | 0.88 | 0.53 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0515000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.274 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1114600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3V8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 37 | 24 | 39 |
| disruptive | 54 | 115 | 55 | 106 |
| missense | 30 | 67 | 44 | 79 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 30 | 73 | 25 | 49 |
| disruptive | 98 | 184 | 96 | 198 |
| missense | 48 | 117 | 68 | 129 |
PlasmoDB Total SNPs: 287
Non-coding: 160 | Synonymous: 74 | Nonsynonymous: 53 | Stop Codon: 0
Protein Length: 848 | Molecular Weight (kDa): 98.383
UniProt ID(s): Q8I3V8
PDB ID(s): None
Isoelectric Point: 5.42
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000504 | RNA recognition motif domain |
| InterPro | IPR032297 | Torus domain |
| InterPro | IPR034181 | Pre-mRNA-splicing factor Cwc2, RNA recognition motif |
| InterPro | IPR035979 | RNA-binding domain superfamily |
| InterPro | IPR039171 | Pre-mRNA-splicing factor Cwc2/Slt11 |
| InterPro | IPR039173 | Pre-mRNA-splicing factor Cwc2 |
| PFam | PF00076 | RNA recognition motif domain |
| PFam | PF16131 | Torus domain |
| Superfamily | SSF54928 | RNA-binding domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 32017676 | The chromatin bound proteome of the human malaria parasite | Batugedara G, Lu XM, ..., Le Roch KG | 10.1099/mgen.0.000327 |
| 33547186 | Saccharomyces cerevisiae Ecm2 Modulates the Catalytic Steps of pre-mRNA Splicing | van der Feltz C, Nikolai B, ..., Hoskins AA | 10.1261/rna.077727.120 |
| 27368340 | A protein map of the yeast activated spliceosome as obtained by electronmicroscopy | Sun C, Rigo N, ..., Luhrmann R | 10.1261/rna.057778.116 |
| 21386897 | Systematic two-hybrid and comparative proteomic analyses reveal novel yeastpre-mRNA splicing factors connected to Prp19 | Ren L, McLean JR, ..., Gould KL | 10.1371/journal.pone.0016719 |