About Gene List

PF3D7_0515100 (ROM9)

Genome location: Pf3D7_05_v3:624,719..626,844(-)

Genome classification: Core

Function and Localization

Product Description: rhomboid protease ROM9

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 3.4.21.105 (Rhomboid protease)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005739 mitochondrion
Function GO:0004252 serine-type endopeptidase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring possibly expressed 0.04 0.98
Trophozoite expressed 0.47 0.72
Schizont possibly expressed 0.14 0.91
Gametocyte expressed 0.42 0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0515100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.258 | MFS: -2.688 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1114700)

Modification: Disrupted | RMgm-3004

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3V7

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.4.21.105 rhomboid protease dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
O67346 Rhomboid domain-containing protein OrthoMCL, OrthoMCL BLAST cid_16094‐Chloro‐7‐nitro‐3‐[(5‐phenylpentyl)oxy]isochromen‐1‐one (21)
P09391 Rhomboid protease GlpG OrthoMCL BLAST CHEMBL4856971

Orthology Information

Ortholog Group (OrthoMCL): OG6_106921

No human ortholog(s)

Protein Information

Protein Length: 488 | Molecular Weight (kDa): 59.16

UniProt ID(s): Q8I3V7

PDB ID(s): None

Isoelectric Point: 10.21

Protein Domain Annotations:

Source Family ID Description
InterPro IPR022764 Peptidase S54, rhomboid domain
PFam PF01694 Peptidase S54, rhomboid domain
Superfamily SSF144091 N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 27 12 25
disruptive 10 43 24 71
missense 8 41 21 59

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 11 46 11 31
disruptive 21 111 51 121
missense 15 87 36 89

PlasmoDB Total SNPs: 161

Non-coding: 121 | Synonymous: 31 | Nonsynonymous: 9 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
34494883 A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. van Esveld SL, Meerstein-Kessel L, ..., Huynen MA 10.1128/mSphere.00614-21
23490234 Loss-of-function analyses defines vital and redundant functions of the Plasmodium rhomboid protease family Lin JW, Meireles P, ..., Khan SM 10.1111/mmi.12187