Product Description: phosphatidylinositol 3-kinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.137 (Phosphatidylinositol 3-kinase);2.7.1.150 (1-phosphatidylinositol-3-phosphate 5-kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0031410 | cytoplasmic vesicle |
| Component | GO:0005768 | endosome |
| Component | GO:0020020 | food vacuole |
| Component | GO:0043656 | host intracellular region |
| Component | GO:0016020 | membrane |
| Component | GO:0005777 | peroxisome |
| Component | GO:0000407 | phagophore assembly site |
| Component | GO:0034271 | phosphatidylinositol 3-kinase complex, class III, type I |
| Component | GO:0034272 | phosphatidylinositol 3-kinase complex, class III, type II |
| Function | GO:0016303 | 1-phosphatidylinositol-3-kinase activity |
| Function | GO:0030492 | hemoglobin binding |
| Function | GO:0052742 | phosphatidylinositol kinase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0000045 | autophagosome assembly |
| Process | GO:0030242 | autophagy of peroxisome |
| Process | GO:0006897 | endocytosis |
| Process | GO:0046907 | intracellular transport |
| Process | GO:0046854 | phosphatidylinositol phosphate biosynthetic process |
| Process | GO:0036092 | phosphatidylinositol-3-phosphate biosynthetic process |
| Process | GO:0048015 | phosphatidylinositol-mediated signaling |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.42 | 0.81 |
| Trophozoite | expressed | 1.48 | 0.34 |
| Schizont | expressed | 0.91 | 0.56 |
| Gametocyte | expressed | 1.03 | 0.49 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0515300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.242 | MFS: -2.674 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1114900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3V5
"Best" AlphaFill ligand hit: BHO (benzhydroxamic acid, Local RMSD=0.06) with 5SXJ (Global RMSD=4.03)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P42336 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform | Homo sapiens | OrthoDB | LY294002imidazopyridine derivative, 3imidazopyridine derivative, 4imidazopyridine derivative, 7a... |
| P42338 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform | Homo sapiens | OrthoDB | 17-Hydroxywortmannin Analogue, 13LY294002PIK-75, 1CHEMBL541643... |
| Q9Z1L0 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform | OrthoDB | US10202371, Example 1US10202371, Example 2US10202371, Example 3US10202371, Example 4... | |
| O00329 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform | Homo sapiens | OrthoDB | US9828378, # 1CID24905148CID24905149PIK293... |
| O75747 | Phosphatidylinositol 3-kinase C2 domain-containing subunit gamma | Homo sapiens | OrthoDB | US11091472, Compound S-1US11091472, Compound S-160US11091472, Compound S-231US11091472, Compound S-261... |
| P32871 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform | Bos taurus | OrthoDB | CHEMBL3589320CHEMBL3589321CHEMBL3589322CHEMBL3589323... |
| Q8NEB9 | Phosphatidylinositol 3-kinase catalytic subunit type 3 | Homo sapiens | OrthoDB | STK16-IN-1US10112926, Example 4US10112926, Example 4.1US10112926, Example 9... |
| Q9JHG7 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform | OrthoDB | GS-9820Cyclosporine ACHEMBL3753671CHEMBL3752369... | |
| D3ZFJ0 | Phosphatidylinositol-4,5-bisphosphate 3-kinase | OrthoDB | CHEMBL4126773 | |
| O00443 | Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha | OrthoDB | STK16-IN-1CHEMBL4166144CHEMBL4160104CHEMBL4167351... | |
| P48736 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform | Homo sapiens | OrthoDB | LY294002med.21724, Compound 4Myricetin (Myr)Staurosporine, 8... |
| Q8BTI9 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform | OrthoDB | GS-9820 | |
| P42337 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform | OrthoDB | US9156831, 1US9156831, 3US9156831, 4US9156831, 5... | |
| O35904 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform | OrthoDB | GS-9820US10202371, Example 1US10202371, Example 2US10202371, Example 3... | |
| A0A0G2K344 | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform | OrthoDB | US10202371, Example 1US10202371, Example 2US10202371, Example 3US10202371, Example 4... | |
| O00750 | Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta | Homo sapiens | OrthoDB | GDC-0941LY294002PIK-75, 1CHEMBL541643... |
Ortholog Group (OrthoMCL): OG6_101850
Most Similar Human Ortholog: A0A0B4J2B1
TM-align score: 0.95 | RMSD: 1.08
Seq Identity: 0.42 | Length: 104 / 2133
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000078142 | phosphatidylinositol 3-kinase catalytic subunit type 3 |
Protein Length: 2133 | Molecular Weight (kDa): 255.921
UniProt ID(s): Q8I3V5
PDB ID(s): None
Isoelectric Point: 9.63
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000403 | Phosphatidylinositol 3-/4-kinase, catalytic domain |
| InterPro | IPR001263 | Phosphoinositide 3-kinase, accessory (PIK) domain |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR015433 | Phosphatidylinositol kinase |
| InterPro | IPR016024 | Armadillo-type fold |
| InterPro | IPR018936 | Phosphatidylinositol 3/4-kinase, conserved site |
| PFam | PF00454 | Phosphatidylinositol 3-/4-kinase, catalytic domain |
| PFam | PF00613 | Phosphoinositide 3-kinase, accessory (PIK) domain |
| Superfamily | SSF48371 | Armadillo-type fold |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 79 | 29 | 91 |
| disruptive | 93 | 245 | 132 | 315 |
| missense | 48 | 154 | 107 | 239 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 39 | 126 | 57 | 122 |
| disruptive | 150 | 466 | 235 | 433 |
| missense | 63 | 325 | 163 | 300 |
PlasmoDB Total SNPs: 338
Non-coding: 21 | Synonymous: 169 | Nonsynonymous: 148 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 20093402 | PfPI3K, a phosphatidylinositol-3 kinase from Plasmodium falciparum, is exportedto the host erythrocyte and is involved in hemoglobin trafficking. | Vaid A, Ranjan R, ..., Sharma P | 10.1182/blood-2009-08-238972 |
| 25874676 | A molecular mechanism of artemisinin resistance in Plasmodium falciparum malaria. | Mbengue A, Bhattacharjee S, ..., Haldar K | 10.1038/nature14412 |
| 32543181 | Heterologous Expression, Purification, and Functional Analysis of the Plasmodiumfalciparum Phosphatidylinositol 4-Kinase IIIbeta. | Sternberg AR, Roepe PD | 10.1021/acs.biochem.0c00259 |