PF3D7_0515500 (GEP1)

Genome location: Pf3D7_05_v3:641,137..644,685(-)

Genome classification: Core

Function and Localization

Product Description: gametogenesis essential protein 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 14

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Process	GO:0006182	cGMP biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.01	1.00
Trophozoite	expressed	0.02	0.99
Schizont	expressed	0.03	0.98
Gametocyte	expressed	0.75	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0515500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.338 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1115100)

Relative Growth Rate: 0.97 ± 0.11
Confidence: 5.79

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_1116300)

Modification: Tagged | RMgm-4820

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3V3

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533580

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	27	20	38
disruptive	31	45	23	83
missense	31	43	22	72

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	57	33	60
disruptive	44	107	53	134
missense	40	89	45	107

PlasmoDB Total SNPs: 203

Non-coding: 134 | Synonymous: 29 | Nonsynonymous: 40 | Stop Codon: 0

Protein Information

Protein Length: 960 | Molecular Weight (kDa): 115.453

UniProt ID(s): Q8I3V3

PDB ID(s): None

Isoelectric Point: 9.91

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
11152613	Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes.	Krogh A, Larsson B, von Heijne G, Sonnhammer EL	10.1006/jmbi.2000.4315
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
32273496	An intracellular membrane protein GEP1 regulates xanthurenic acid inducedgametogenesis of malaria parasites.	Jiang Y, Wei J, ..., Yuan J	10.1038/s41467-020-15479-3
2111230	A genetic analysis of glucoamylase activity in the diastatic yeast Saccharomycescerevisiae NCYC 625	Patel D, Evans IH, Bevan EA	10.1007/BF00314873
34361604	Structural Characterization of a Polysaccharide from Gastrodia elata and ItsBioactivity on Gut Microbiota	Huo J, Lei M, ..., Wu W	10.3390/molecules26154443
36432165	Structural Characterization of Polysaccharide Derived from Gastrodia elata andIts Immunostimulatory Effect on RAW264.7 Cells	Guan H, Ling X, ..., Liu X	10.3390/molecules27228059
36768882	Characterizing the Specific Recognition of Xanthurenic Acid by GEP1 and GEP1-GCalphaInteractions in cGMP Signaling Pathway in Gametogenesis of Malaria Parasites	Zhu C, Liang X, ..., Luo S	10.3390/ijms24032561