Product Description: glideosome-associated protein 40
SignalP Peptide: N/A
# Transmembrane Domains: 10
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0071944 | cell periphery |
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0070258 | inner membrane pellicle complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Process | GO:0071976 | cell gliding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.24 | 0.85 |
| Schizont | expressed | 1.68 | 0.30 |
| Gametocyte | expressed | 0.55 | 0.69 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0515700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.994 | MFS: -1.939 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1115300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1115300)
Modification: Tagged | RMgm-4554
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3V1
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 19 | 10 | 29 |
| disruptive | 5 | 12 | 3 | 22 |
| missense | 4 | 11 | 3 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 37 | 23 | 28 |
| disruptive | 7 | 29 | 28 | 100 |
| missense | 6 | 23 | 21 | 78 |
PlasmoDB Total SNPs: 165
Non-coding: 155 | Synonymous: 8 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 456 | Molecular Weight (kDa): 51.8
UniProt ID(s): Q8I3V1
PDB ID(s): None
Isoelectric Point: 7.91
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 20951968 | Functional dissection of the apicomplexan glideosome molecular architecture. | Frenal K, Polonais V, ..., Soldati-Favre D | 10.1016/j.chom.2010.09.002 |
| 26292257 | Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for VaccineDevelopment. | Aguiar JC, Bolton J, ..., Richie TL | 10.1371/journal.pone.0136109 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 21610105 | Unusual N-glycan structures required for trafficking Toxoplasma gondii GAP50 tothe inner membrane complex regulate host cell entry through parasite motility | Fauquenoy S, Hovasse A, ..., Tomavo S | 10.1074/mcp.M111.008953 |
| 26845335 | Gliding Associated Proteins Play Essential Roles during the Formation of theInner Membrane Complex of Toxoplasma gondii | Harding CR, Egarter S, ..., Meissner M | 10.1371/journal.ppat.1005403 |
| 25393004 | Plasticity between MyoC- and MyoA-glideosomes: an example of functionalcompensation in Toxoplasma gondii invasion | Frenal K, Marq JB, ..., Soldati-Favre D | 10.1371/journal.ppat.1004504 |
| 30130043 | [Research Advances on Gliding-associated Proteins of Toxoplasma gondii] | Li RH, Yin GR | https://pubmed.ncbi.nlm.nih.gov/30130043/ |
| 37249423 | Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion andGametocytogenesis | He L, Qiu Y, ..., Zhu X | 10.1128/spectrum.01434-23 |
| 28893907 | Compositional and expression analyses of the glideosome during the Plasmodiumlife cycle reveal an additional myosin light chain required for maximum motility | Green JL, Wall RJ, ..., Holder AA | 10.1074/jbc.M117.802769 |
| 24779470 | Assessment of phosphorylation in Toxoplasma glideosome assembly and function | Jacot D, Frenal K, ..., Soldati-Favre D | 10.1111/cmi.12307 |
| 16321976 | A conserved molecular motor drives cell invasion and gliding motility acrossmalaria life cycle stages and other apicomplexan parasites | Baum J, Richard D, ..., Cowman AF | 10.1074/jbc.M509807200 |