Product Description: NLI interacting factor-like phosphatase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0004721 | phosphoprotein phosphatase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.39 | 0.75 |
| Schizont | expressed | 0.45 | 0.71 |
| Gametocyte | expressed | 0.27 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0515900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.645 | MFS: -2.94 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1403300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1115500)
Modification: Disrupted | RMgm-3010
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3U9
"Best" AlphaFill ligand hit: KEG (12-tungstophosphate, Local RMSD=0.32) with 2HHL (Global RMSD=1.85)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9GZU7 | Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 | Homo sapiens | OrthoDB | MLS000070184MLS000068938MLS000031372MLS000073937... |
Ortholog Group (OrthoMCL): OG6_100959
Most Similar Human Ortholog: F8W184
TM-align score: 0.88 | RMSD: 1.98
Seq Identity: 0.29 | Length: 119 / 328
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000144579 | CTD small phosphatase 1 |
| ENSG00000144677 | CTD small phosphatase like |
| ENSG00000175215 | CTD small phosphatase 2 |
Protein Length: 328 | Molecular Weight (kDa): 39.75
UniProt ID(s): Q8I3U9
PDB ID(s): None
Isoelectric Point: 10.29
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004274 | FCP1 homology domain |
| InterPro | IPR011948 | Dullard phosphatase domain, eukaryotic |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF03031 | FCP1 homology domain |
| Superfamily | SSF56784 | HAD-like superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 13 | 11 | 21 |
| disruptive | 0 | 9 | 5 | 23 |
| missense | 0 | 9 | 4 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 28 | 12 | 21 |
| disruptive | 5 | 37 | 26 | 104 |
| missense | 1 | 24 | 20 | 43 |
PlasmoDB Total SNPs: 82
Non-coding: 72 | Synonymous: 8 | Nonsynonymous: 2 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |