Genome location: Pf3D7_05_v3:667,396..675,244(+)
Genome classification: Core
Product Description: cation-transporting ATPase 1
SignalP Peptide: N/A
# Transmembrane Domains: 10
EC Numbers: 3.6.3.12 (Transferred entry: 7.2.2.6);7.2.2.- (Linked to the hydrolysis of a nucleoside triphosphate.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005886 | plasma membrane |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.40 | 0.77 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.61 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0516100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.696 | #Insertions: 6
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1103600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3U7
"Best" AlphaFill ligand hit: PCW ((z,z)-4-hydroxy-n,n,n-trimethyl-10-oxo-7-[(1-oxo-9-octadecenyl)oxy]-3,5,9-trioxa-4-phosphaheptacos-18-en-1-aminium-4-oxide, Local RMSD=0.02) with 7D94 (Global RMSD=3.83)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100379
Most Similar Human Ortholog: Q9NQ11
TM-align score: 0.82 | RMSD: 4.56
Seq Identity: 0.26 | Length: 1088 / 2400
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000127249 | ATPase 13A4 |
| ENSG00000133657 | ATPase 13A3 |
| ENSG00000159363 | ATPase cation transporting 13A2 |
| ENSG00000187527 | ATPase 13A5 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 80 | 36 | 117 |
| disruptive | 118 | 267 | 127 | 420 |
| missense | 75 | 202 | 104 | 339 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 35 | 156 | 80 | 138 |
| disruptive | 204 | 533 | 280 | 532 |
| missense | 112 | 405 | 233 | 398 |
PlasmoDB Total SNPs: 384
Non-coding: 82 | Synonymous: 165 | Nonsynonymous: 137 | Stop Codon: 0
Protein Length: 2400 | Molecular Weight (kDa): 283.6
PDB ID(s): None
Isoelectric Point: 9.34
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001757 | P-type ATPase |
| InterPro | IPR006544 | P-type ATPase, subfamily V |
| InterPro | IPR008250 | P-type ATPase, A domain superfamily |
| InterPro | IPR018303 | P-type ATPase, phosphorylation site |
| InterPro | IPR023298 | P-type ATPase, transmembrane domain superfamily |
| InterPro | IPR023299 | P-type ATPase, cytoplasmic domain N |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF00122 | P-type ATPase, subfamily V |
| PFam | PF12409 | |
| Superfamily | SSF56784 | HAD-like superfamily |
| Superfamily | SSF81653 | P-type ATPase, A domain superfamily |
| Superfamily | SSF81660 | P-type ATPase, cytoplasmic domain N |
| Superfamily | SSF81665 | P-type ATPase, transmembrane domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 8421054 | A family of cation ATPase-like molecules from Plasmodium falciparum. | Krishna S, Cowan G, ..., Robson KJ | 10.1083/jcb.120.2.385 |
| 11152613 | Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. | Krogh A, Larsson B, von Heijne G, Sonnhammer EL | 10.1006/jmbi.2000.4315 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 33485966 | Aminoglycerophospholipid flipping and P4-ATPases in Toxoplasma gondii | Chen K, Gunay-Esiyok O, ..., Gupta N | 10.1016/j.jbc.2021.100315 |
| 36936814 | Apically-located P4-ATPase1-Lem1 complex internalizes phosphatidylserine andregulates motility-dependent invasion and egress in Toxoplasma gondii | Chen K, Huang X, ..., Gupta N | 10.1016/j.csbj.2023.02.032 |