Product Description: tRNA pseudouridine synthase, putative
SignalP Peptide: MNKFFPLLVCVSLIFFFLKLSINA
# Transmembrane Domains: 1
EC Numbers: 4.2.1.70 (Pseudouridylate synthase);5.4.99.12 (tRNA pseudouridine(38-40) synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0009982 | pseudouridine synthase activity |
| Process | GO:1990481 | mRNA pseudouridine synthesis |
| Process | GO:0031119 | tRNA pseudouridine synthesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.21 | 0.88 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.24 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0516300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.998 | MFS: -2.549 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1231100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1231100)
Modification: Disrupted | RMgm-3322
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3U5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 4.2.1.70 | pseudouridylate synthase | tRNAiodoacetamide5-fluorouracilthiol reagents1,10-phenanthrolinep-chloromercuribenzoate1-(Tetrahydro-2-furanyl)-5-fluorouracil |
| 5.4.99.12 | tRNA pseudouridine38-40 synthase | tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 30 | 9 | 42 |
| disruptive | 15 | 59 | 31 | 85 |
| missense | 10 | 55 | 30 | 76 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 59 | 32 | 34 |
| disruptive | 28 | 137 | 66 | 157 |
| missense | 16 | 117 | 51 | 97 |
PlasmoDB Total SNPs: 192
Non-coding: 101 | Synonymous: 41 | Nonsynonymous: 50 | Stop Codon: 0
Protein Length: 681 | Molecular Weight (kDa): 78.344
UniProt ID(s): Q8I3U5
PDB ID(s): None
Isoelectric Point: 8.1
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001406 | Pseudouridine synthase I, TruA |
| InterPro | IPR020097 | Pseudouridine synthase I, TruA, alpha/beta domain |
| InterPro | IPR020103 | Pseudouridine synthase, catalytic domain superfamily |
| PFam | PF01416 | Pseudouridine synthase I, TruA, alpha/beta domain |
| Superfamily | SSF55120 | Pseudouridine synthase, catalytic domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |