PF3D7_0516700

Genome location: Pf3D7_05_v3:693,120..699,060(+)

Genome classification: Core

Function and Localization

Product Description: ubiquitin carboxyl-terminal hydrolase 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004843	thiol-dependent deubiquitinase
Process	GO:0016579	protein deubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.48	0.76
Trophozoite	not expressed	0.61	0.66
Schizont	not expressed	1.52	0.30
Gametocyte	not expressed	0.42	0.76

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0516700

Resistome Missense Mutations: L618Q

Resistome Compounds with Missense Mutations: BCH070

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.613 | MFS: -1.588 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1231500)

Relative Growth Rate: 0.83 ± 0.15
Confidence: 5.20

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1231500)

Modification: Disrupted | RMgm-3324

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3U1

"Best" AlphaFill ligand hit: MIX (mitoxantrone, Local RMSD=0.03) with 7R2G (Global RMSD=3.14)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.19.12	ubiquitinyl hydrolase 1	KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
O94966	Ubiquitin carboxyl-terminal hydrolase 19		OrthoDB	US11053213, Example 7US11053213, Example 14US11053213, Example 25US11053213, Example 31...
Q13107	Ubiquitin carboxyl-terminal hydrolase 4	Homo sapiens	OrthoDB	VIALININ ACHEMBL4593739

Orthology Information

Ortholog Group (OrthoMCL): OG6_101021

Most Similar Human Ortholog: O75604

TM-align score: 0.55 | RMSD: 3.28

Seq Identity: 0.33 | Length: 363 / 1332

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000036672	ubiquitin specific peptidase 2
ENSG00000138592	ubiquitin specific peptidase 8

Protein Information

Protein Length: 1332 | Molecular Weight (kDa): 156.332

UniProt ID(s): Q8I3U1

PDB ID(s): None

Isoelectric Point: 6.78

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001394	Peptidase C19, ubiquitin carboxyl-terminal hydrolase
InterPro	IPR018200	Ubiquitin specific protease, conserved site
InterPro	IPR038765	Papain-like cysteine peptidase superfamily
PFam	PF00443	Peptidase C19, ubiquitin carboxyl-terminal hydrolase
Superfamily	SSF54001	Papain-like cysteine peptidase superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	17	43	39	67
disruptive	38	87	35	122
missense	16	63	30	95

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	23	110	56	65
disruptive	69	168	89	229
missense	34	113	69	169

PlasmoDB Total SNPs: 298

Non-coding: 173 | Synonymous: 71 | Nonsynonymous: 54 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104