PF3D7_0516900 (RPL2)

Genome location: Pf3D7_05_v3:712,371..714,307(-)

Genome classification: Core

Function and Localization

Product Description: 60S ribosomal protein L2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0022625	cytosolic large ribosomal subunit
Component	GO:0005634	nucleus
Function	GO:0003723	RNA binding
Function	GO:0003735	structural constituent of ribosome
Process	GO:0002181	cytoplasmic translation
Process	GO:0006412	translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.65	0.38
Trophozoite	expressed	2.05	0.21
Schizont	expressed	0.45	0.74
Gametocyte	expressed	1.08	0.47

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0516900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.979 | MFS: -1.816 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3T9

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100234

Most Similar Human Ortholog: E9PKZ0

TM-align score: 0.99 | RMSD: 0.58

Seq Identity: 0.65 | Length: 205 / 260

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000112651	mitochondrial ribosomal protein L2
ENSG00000161016	ribosomal protein L8
ENSG00000288564	novel protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	13	8	16
disruptive	1	2	1	32
missense	1	2	1	31

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	29	8	14
disruptive	5	14	24	73
missense	2	8	23	57

PlasmoDB Total SNPs: 86

Non-coding: 82 | Synonymous: 2 | Nonsynonymous: 1 | Stop Codon: 1

Protein Information

Protein Length: 260 | Molecular Weight (kDa): 28

UniProt ID(s): Q8I3T9

PDB ID(s): 3J79, 3JBN, 3JBO, 3JBP, 5UMD

Isoelectric Point: 11.18

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002171	Ribosomal protein L2
InterPro	IPR008991	Translation protein SH3-like domain superfamily
InterPro	IPR012340	Nucleic acid-binding, OB-fold
InterPro	IPR022666	Ribosomal Proteins L2, RNA binding domain
InterPro	IPR022669	Ribosomal protein L2, C-terminal
InterPro	IPR022671	Ribosomal protein L2, conserved site
PFam	PF00181	Ribosomal Proteins L2, RNA binding domain
PFam	PF03947	Ribosomal protein L2, C-terminal
Superfamily	SSF50104	Translation protein SH3-like domain superfamily
Superfamily	SSF50249	Nucleic acid-binding, OB-fold

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
24913268	Cryo-E M structure of the Plasmodium falciparum 80 S ribosome bound to theanti-protozoan drug emetine	Wong W, Bai XC, ..., Scheres SH	10.7554/eLife.03080
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596
33906926	Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH	Sinha A, Baumgarten S, ..., Scherf A	10.1128/mBio.00661-21