Product Description: FACT complex subunit SPT16, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0035101 | FACT complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0031491 | nucleosome binding |
| Process | GO:0034724 | DNA replication-independent chromatin organization |
| Process | GO:0032968 | positive regulation of transcription elongation from RNA polymerase II promoter |
| Process | GO:0006368 | transcription elongation from RNA polymerase II promoter |
| Process | GO:0006351 | transcription, DNA-templated |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.45 | 0.78 |
| Trophozoite | expressed | 1.11 | 0.44 |
| Schizont | expressed | 0.38 | 0.77 |
| Gametocyte | expressed | 1.01 | 0.50 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0517400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -2.94 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1232200)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1232200)
Modification: Tagged | RMgm-655
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3T4
"Best" AlphaFill ligand hit: UMP (2'-deoxyuridine 5'-monophosphate, Local RMSD=0.10) with 7S01 (Global RMSD=24.61)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102309
Most Similar Human Ortholog: G3V401
TM-align score: 0.75 | RMSD: 2.35
Seq Identity: 0.17 | Length: 80 / 1141
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000092201 | SPT16 homolog, facilitates chromatin remodeling subunit |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 38 | 21 | 50 |
| disruptive | 69 | 117 | 49 | 128 |
| missense | 34 | 48 | 26 | 82 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 27 | 72 | 38 | 68 |
| disruptive | 105 | 202 | 71 | 189 |
| missense | 42 | 104 | 45 | 111 |
PlasmoDB Total SNPs: 187
Non-coding: 81 | Synonymous: 50 | Nonsynonymous: 56 | Stop Codon: 0
Protein Length: 1141 | Molecular Weight (kDa): 132.682
PDB ID(s): None
Isoelectric Point: 4.61
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000994 | Peptidase M24 |
| InterPro | IPR013719 | Domain of unknown function DUF1747 |
| InterPro | IPR013953 | FACT complex subunit Spt16 domain |
| InterPro | IPR029148 | FACT complex subunit Spt16, N-terminal lobe domain |
| InterPro | IPR036005 | Creatinase/aminopeptidase-like |
| InterPro | IPR040258 | FACT complex subunit Spt16 |
| PFam | PF00557 | Peptidase M24 |
| PFam | PF08512 | Domain of unknown function DUF1747 |
| PFam | PF08644 | FACT complex subunit Spt16 domain |
| PFam | PF14826 | FACT complex subunit Spt16, N-terminal lobe domain |
| Superfamily | SSF55920 | Creatinase/aminopeptidase-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21899698 | Experimentally controlled downregulation of the histone chaperone FACT inPlasmodium berghei reveals that it is critical to male gamete fertility. | Laurentino EC, Taylor S, ..., Waters AP | 10.1111/j.1462-5822.2011.01683.x |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |