PF3D7_0518000

Genome location: Pf3D7_05_v3:750,421..752,625(+)

Genome classification: Core

Function and Localization

Product Description: NOSIP domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.31	0.84
Trophozoite	expressed	0.36	0.79
Schizont	expressed	0.26	0.83
Gametocyte	expressed	0.74	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0518000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.42 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1232800)

Relative Growth Rate: 1.04 ± 0.22
Confidence: 4.39

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1232800)

Modification: Disrupted | RMgm-3334

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4E6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103091

Most Similar Human Ortholog: Q9Y314

TM-align score: 0.77 | RMSD: 3.28

Seq Identity: 0.23 | Length: 244 / 270

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000142546	nitric oxide synthase interacting protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	14	12	23
disruptive	3	21	11	40
missense	3	21	9	34

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	36	16	23
disruptive	11	64	20	90
missense	3	53	15	63

PlasmoDB Total SNPs: 176

Non-coding: 158 | Synonymous: 14 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 270 | Molecular Weight (kDa): 31.472

UniProt ID(s): C0H4E6

PDB ID(s): None

Isoelectric Point: 10.02

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR016818	Nitric oxide synthase-interacting protein
InterPro	IPR031790	Nitric oxide synthase-interacting protein, zinc-finger
PFam	PF15906	Nitric oxide synthase-interacting protein, zinc-finger
Superfamily	SSF57850	N/A