PF3D7_0518300

Genome location: Pf3D7_05_v3:759,377..761,483(-)

Genome classification: Core

Function and Localization

Product Description: proteasome subunit beta type-1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.06	0.97
Trophozoite	expressed	0.28	0.83
Schizont	expressed	0.13	0.90
Gametocyte	expressed	0.12	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0518300

Resistome Missense Mutations: A117V

Resistome Compounds with Missense Mutations: WLL

Resistome # Samples with Disruptive Mutations: 2 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.135 | MFS: -3.04 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1233100)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K7U1

"Best" AlphaFill ligand hit: PHQ (benzyl chlorocarbonate, Local RMSD=0.11) with 5LF6 (Global RMSD=0.83)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.25.1	proteasome endopeptidase complex	ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P20618	Proteasome subunit beta type-1	Homo sapiens	OMA, OrthoDB	CHEMBL2385817CHEMBL3287937CHEMBL3287939CHEMBL3287940...
P23724	Proteasome subunit beta type-6	Saccharomyces cerevisiae	OMA, OrthoDB, OrthoMCL	Apam-YET

Orthology Information

Ortholog Group (OrthoMCL): OG6_101631

Most Similar Human Ortholog: P20618

TM-align score: 0.89 | RMSD: 1.17

Seq Identity: 0.42 | Length: 218 / 240

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000008018	proteasome 20S subunit beta 1

Protein Information

Protein Length: 240 | Molecular Weight (kDa): 27.268

UniProt ID(s): A0A5K1K7U1

PDB ID(s): 5FMG, 7LXT, 7LXU

Isoelectric Point: 7.02

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001353	Proteasome, subunit alpha/beta
InterPro	IPR016050	Proteasome beta-type subunit, conserved site
InterPro	IPR029055	Nucleophile aminohydrolases, N-terminal
InterPro	IPR035202	Proteasome subunit beta 1
PFam	PF00227	Proteasome, subunit alpha/beta
Superfamily	SSF56235	Nucleophile aminohydrolases, N-terminal

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	13	7	10
disruptive	1	5	6	20
missense	1	4	5	17

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	25	5	21
disruptive	2	22	27	59
missense	1	17	25	40

PlasmoDB Total SNPs: 154

Non-coding: 134 | Synonymous: 16 | Nonsynonymous: 4 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
30379851	Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation.	Briquet S, Ourimi A, ..., Vaquero C	10.1371/journal.pone.0205596