PF3D7_0518500 (DDX23)

Genome location: Pf3D7_05_v3:762,933..767,061(-)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DDX23, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.62	0.72
Trophozoite	expressed	0.93	0.51
Schizont	expressed	0.28	0.83
Gametocyte	expressed	0.95	0.50

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0518500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.515 | MFS: -2.462 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1233300)

Relative Growth Rate: 0.10 ± 0.10
Confidence: 5.97

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K868

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.11) with 6L5N (Global RMSD=1.87)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102378

Most Similar Human Ortholog: Q9BUQ8

TM-align score: 0.69 | RMSD: 3.37

Seq Identity: 0.48 | Length: 609 / 1123

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000174243	DEAD-box helicase 23

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	54	32	64
disruptive	61	174	80	192
missense	28	103	63	125

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	22	117	47	68
disruptive	97	342	153	315
missense	42	222	105	190

PlasmoDB Total SNPs: 183

Non-coding: 53 | Synonymous: 71 | Nonsynonymous: 59 | Stop Codon: 0

Protein Information

Protein Length: 1123 | Molecular Weight (kDa): 131.163

UniProt ID(s): A0A5K1K868

PDB ID(s): None

Isoelectric Point: 7.15

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000629	ATP-dependent RNA helicase DEAD-box, conserved site
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase