Product Description: mRNA-binding protein PUF1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0003729 | mRNA binding |
| Process | GO:0010608 | posttranscriptional regulation of gene expression |
| Process | GO:0043487 | regulation of RNA stability |
| Process | GO:0006417 | regulation of translation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.06 | 0.97 |
| Trophozoite | not expressed | 0.07 | 0.96 |
| Schizont | possibly expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 1.05 | 0.48 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0518700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.712 | #Insertions: 4
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1233500)
Modification: Disrupted | RMgm-616
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3S3
"Best" AlphaFill ligand hit: DTD (dithiane diol, Local RMSD=2.28) with 3GVO (Global RMSD=4.97)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 66 | 52 | 90 |
| disruptive | 37 | 136 | 83 | 232 |
| missense | 23 | 112 | 68 | 189 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 159 | 65 | 107 |
| disruptive | 77 | 381 | 225 | 471 |
| missense | 37 | 285 | 180 | 298 |
PlasmoDB Total SNPs: 273
Non-coding: 107 | Synonymous: 101 | Nonsynonymous: 65 | Stop Codon: 0
Protein Length: 1894 | Molecular Weight (kDa): 224.087
PDB ID(s): None
Isoelectric Point: 8.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001313 | Pumilio RNA-binding repeat |
| InterPro | IPR016024 | Armadillo-type fold |
| InterPro | IPR033712 | Pumilio, RNA binding domain |
| PFam | PF00806 | Pumilio RNA-binding repeat |
| Superfamily | SSF48371 | Armadillo-type fold |
| Superfamily | SSF57997 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 12409450 | The malaria parasite Plasmodium falciparum encodes members of the Puf RNA-bindingprotein family with conserved RNA binding activity. | Cui L, Fan Q, Li J | 10.1093/nar/gkf600 |
| 27383769 | The RNA-binding protein Puf1 functions in the maintenance of gametocytes inPlasmodium falciparum. | Shrestha S, Li X, ..., Cui L | 10.1242/jcs.186908 |
| 28596535 | Identification and characterization of roles for Puf1 and Puf2 proteins in theyeast response to high calcium | Haramati O, Brodov A, ..., Arava Y | 10.1038/s41598-017-02873-z |
| 21673790 | The Puf-family RNA-binding protein Puf2 controls sporozoite conversion to liverstages in the malaria parasite | Muller K, Matuschewski K, Silvie O | 10.1371/journal.pone.0019860 |
| 21251930 | Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodiumfalciparum transfection | Ganesan SM, Morrisey JM, ..., Vaidya AB | 10.1016/j.molbiopara.2011.01.004 |
| 38148574 | The Plasmodium falciparum CCCH zinc finger protein MD3 regulates malegametocytogenesis through its interaction with RNA-binding proteins | Farrukh A, Musabyimana JP, ..., Ngwa CJ | 10.1111/mmi.15215 |
| 29197418 | Tagging to endogenous genes of Plasmodium falciparum using CRISPR/Cas9 | Kuang D, Qiao J, ..., Dai X | 10.1186/s13071-017-2539-0 |