PF3D7_0519200

Genome location: Pf3D7_05_v3:791,256..793,280(-)

Genome classification: Core

Function and Localization

Product Description: V-type proton ATPase 16 kDa proteolipid subunit

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016021	integral component of membrane
Component	GO:0016020	membrane
Function	GO:0046961	proton-transporting ATPase activity, rotational mechanism
Process	GO:1902600	proton transmembrane transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.08	0.96
Trophozoite	expressed	0.16	0.89
Schizont	expressed	0.14	0.90
Gametocyte	expressed	0.67	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0519200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.776 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1234000)

Relative Growth Rate: 0.05 ± 0.15
Confidence: 5.18

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3R7

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.3.14	H+-transporting two-sector ATPase	No BRENDA inhibitors

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P32842	V-type proton ATPase subunit c'	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	HOGENOM, OrthoDB, OrthoMCL	MLS000684518MLS000723546MLS000684518MLS001195205...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100711

Most Similar Human Ortholog: H3BNI4

TM-align score: 0.97 | RMSD: 0.76

Seq Identity: 0.57 | Length: 112 / 165

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000185883	ATPase H+ transporting V0 subunit c

Protein Information

Protein Length: 165 | Molecular Weight (kDa): 17.065

UniProt ID(s): Q5IHV7, Q8I3R7

PDB ID(s): None

Isoelectric Point: 8.24

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000245	V-ATPase proteolipid subunit
InterPro	IPR002379	V-ATPase proteolipid subunit C-like domain
InterPro	IPR011555	V-ATPase proteolipid subunit C, eukaryotic
InterPro	IPR035921	F/V-ATP synthase subunit C superfamily
PFam	PF00137	V-ATPase proteolipid subunit C-like domain
Superfamily	SSF81333	F/V-ATP synthase subunit C superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	8	3	3
disruptive	6	1	1	3
missense	6	1	1	3

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	16	3	7
disruptive	9	6	9	31
missense	8	3	7	25

PlasmoDB Total SNPs: 224

Non-coding: 206 | Synonymous: 13 | Nonsynonymous: 5 | Stop Codon: 0