Product Description: protoheme IX farnesyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 4
EC Numbers: 2.5.1.141 (Heme o synthase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004311 | farnesyltranstransferase activity |
| Function | GO:0004659 | prenyltransferase activity |
| Process | GO:0045333 | cellular respiration |
| Process | GO:0006783 | heme biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.97 |
| Trophozoite | expressed | 0.26 | 0.83 |
| Schizont | expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.24 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0519300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.175 | MFS: -3.179 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I0W6
"Best" AlphaFill ligand hit: MPG ([(z)-octadec-9-enyl] (2r)-2,3-bis(oxidanyl)propanoate, Local RMSD=0.02) with 6M31 (Global RMSD=3.47)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.5.1.141 | heme o synthase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101203
Most Similar Human Ortholog: J3KRW4
TM-align score: 0.65 | RMSD: 2.31
Seq Identity: 0.06 | Length: 53 / 648
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000006695 | cytochrome c oxidase assembly factor heme A:farnesyltransferase COX10 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 19 | 15 | 44 |
| disruptive | 15 | 61 | 39 | 104 |
| missense | 11 | 51 | 37 | 83 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 49 | 30 | 47 |
| disruptive | 28 | 136 | 68 | 137 |
| missense | 16 | 111 | 54 | 93 |
PlasmoDB Total SNPs: 160
Non-coding: 69 | Synonymous: 65 | Nonsynonymous: 26 | Stop Codon: 0
Protein Length: 648 | Molecular Weight (kDa): 75.192
UniProt ID(s): Q8I0W6
PDB ID(s): None
Isoelectric Point: 10.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000537 | UbiA prenyltransferase family |
| InterPro | IPR006369 | Protohaem IX farnesyltransferase |
| InterPro | IPR030470 | UbiA prenyltransferase conserved site |
| PFam | PF01040 | UbiA prenyltransferase family |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 31848371 | Biosynthesis of heme O in intraerythrocytic stages of Plasmodium falciparum andpotential inhibitors of this pathway. | Simao-Gurge RM, Wunderlich G, ..., Katzin AM | 10.1038/s41598-019-55506-y |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 36243715 | Identification of acetic acid sensitive strains through biosensor-based screeningof a Saccharomyces cerevisiae CRISPRi library | Mormino M, Lenitz I, Siewers V, Nygard Y | 10.1186/s12934-022-01938-7 |