Product Description: phosphomethylpyrimidine kinase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.49 (Hydroxymethylpyrimidine kinase);2.7.4.7 (Phosphooxymethylpyrimidine kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Function | GO:0008902 | hydroxymethylpyrimidine kinase activity |
| Function | GO:0016301 | kinase activity |
| Function | GO:0008972 | phosphomethylpyrimidine kinase activity |
| Process | GO:0009228 | thiamine biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.08 | 0.94 |
| Schizont | expressed | 0.07 | 0.94 |
| Gametocyte | expressed | 0.03 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0520500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -0.277 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3Q5
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.13) with 2I5B (Global RMSD=3.41)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.49 | hydroxymethylpyrimidine kinase | pyridoxinerugulactone2-methyl-4-amino-5-hydroxymethylpyrimidine(6R)-6-[(2E)-4-oxo-6-phenylhex-2-en-1-yl]-5,6-dihydro-2H-pyran-2-one(6S)-6-[(2E)-6-(4-ethynylphenyl)-4-oxohex-2-en-1-yl]tetrahydro-2H-pyran-2-one(6R)-6-[(2E)-6-(4-ethynylphenyl)-4-oxohex-2-en-1-yl]-5,6-dihydro-2H-pyran-2-one |
| 2.7.4.7 | phosphooxymethylpyrimidine kinase | p-hydroxymercuribenzoate(6R)-6-[(2E)-4-oxo-6-phenylhex-2-en-1-yl]-5,6-dihydro-2H-pyran-2-one(6S)-6-[(2E)-6-(4-ethynylphenyl)-4-oxohex-2-en-1-yl]tetrahydro-2H-pyran-2-one(6R)-6-[(2E)-6-(4-ethynylphenyl)-4-oxohex-2-en-1-yl]-5,6-dihydro-2H-pyran-2-one |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9HAZ1 | Dual specificity protein kinase CLK4 | Homo sapiens | OrthoDB | CHEMBL3297832CHEMBL3775393CHEMBL3774448CHEMBL3815054... |
| P49759 | Dual specificity protein kinase CLK1 | Homo sapiens | OrthoDB | US8546404, 6NR93A55E4... |
| P49760 | Dual specificity protein kinase CLK2 | Homo sapiens | OrthoDB | US9040694, 2US9040694, 3pyrazolo[1,5-a]pyrimidine 4bDorsomorphin... |
| O35492 | Dual specificity protein kinase CLK3 | Mus musculus | OrthoDB | CHEMBL1803078CHEMBL1803082CHEMBL1803083CHEMBL1803085... |
| O35491 | Dual specificity protein kinase CLK2 | Mus musculus | OrthoDB | CHEMBL3589660CHEMBL3589661CHEMBL3589662CHEMBL3589664... |
| O35493 | Dual specificity protein kinase CLK4 | Mus musculus | OrthoDB | CHEMBL3589660CHEMBL3589661CHEMBL3589662CHEMBL3589664... |
| P22518 | Dual specificity protein kinase CLK1 | Mus musculus | OrthoDB | MLS000518915CHEMBL2029615CHEMBL2029618CHEMBL4784564... |
| P49761 | Dual specificity protein kinase CLK3 | OrthoDB | 3A5CHEMBL3297832CHEMBL3774448CHEMBL3885335... |
Protein Length: 310 | Molecular Weight (kDa): 34.975
UniProt ID(s): Q8I3Q5
PDB ID(s): None
Isoelectric Point: 6.57
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004399 | Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase |
| InterPro | IPR013749 | Pyridoxamine kinase/Phosphomethylpyrimidine kinase |
| InterPro | IPR029056 | Ribokinase-like |
| PFam | PF08543 | Pyridoxamine kinase/Phosphomethylpyrimidine kinase |
| Superfamily | SSF53613 | Ribokinase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 16 | 9 | 17 |
| disruptive | 0 | 12 | 6 | 20 |
| missense | 0 | 12 | 6 | 19 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 26 | 15 | 21 |
| disruptive | 4 | 25 | 29 | 48 |
| missense | 4 | 19 | 23 | 35 |
PlasmoDB Total SNPs: 32
Non-coding: 20 | Synonymous: 11 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |