Product Description: bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.1.17 (Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0033644 | host cell membrane |
| Function | GO:0004081 | bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity |
| Process | GO:0006167 | AMP biosynthetic process |
| Process | GO:0006754 | ATP biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.73 | 0.66 |
| Trophozoite | expressed | 0.50 | 0.72 |
| Schizont | expressed | 0.08 | 0.95 |
| Gametocyte | possibly expressed | 0.26 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0520600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.129 | MFS: -3.063 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4F3
"Best" AlphaFill ligand hit: GPP (geranyl diphosphate, Local RMSD=2.19) with 6YPF (Global RMSD=5.22)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.1.17 | bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_104771
Most Similar Human Ortholog: P50583
TM-align score: 0.91 | RMSD: 1.77
Seq Identity: 0.35 | Length: 146 / 152
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000164978 | nudix hydrolase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 8 | 7 | 14 |
| disruptive | 3 | 13 | 5 | 22 |
| missense | 3 | 13 | 5 | 22 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 23 | 4 | 13 |
| disruptive | 7 | 22 | 13 | 40 |
| missense | 7 | 20 | 13 | 31 |
PlasmoDB Total SNPs: 96
Non-coding: 84 | Synonymous: 8 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 152 | Molecular Weight (kDa): 17.75
UniProt ID(s): C0H4F3
Isoelectric Point: 5.69
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000086 | NUDIX hydrolase domain |
| InterPro | IPR003565 | Bis(5'-nucleosyl)-tetraphosphatase |
| InterPro | IPR015797 | NUDIX hydrolase-like domain superfamily |
| InterPro | IPR020084 | NUDIX hydrolase, conserved site |
| PFam | PF00293 | NUDIX hydrolase domain |
| Superfamily | SSF55811 | NUDIX hydrolase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 22791172 | Molecular characterization and mutational analysis of recombinant diadenosine5',5''-P(1),P(4)-tetraphosphate hydrolase from Plasmodium falciparum. | Osman W, Endo S, ..., Kitade Y | 10.1248/bpb.b12-00165 |
| 26829485 | Structural and functional attributes of malaria parasite diadenosinetetraphosphate hydrolase. | Sharma A, Yogavel M, Sharma A | 10.1038/srep19981 |
| 30700216 | Functional genetic evaluation of DNA house-cleaning enzymes in the malariaparasite: dUTPase and Ap4AH are essential in Plasmodium berghei but ITPase andNDH are dispensable | Kumar H, Kehrer J, ..., Frischknecht F | 10.1080/14728222.2019.1575810 |