Product Description: adenosylhomocysteinase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.3.1.1 (Adenosylhomocysteinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005829 | cytosol |
| Component | GO:0005622 | intracellular |
| Function | GO:0004013 | adenosylhomocysteinase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0033353 | S-adenosylmethionine cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.66 | 0.70 |
| Trophozoite | expressed | 2.21 | 0.18 |
| Schizont | expressed | 0.94 | 0.56 |
| Gametocyte | expressed | 0.35 | 0.82 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0520900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.194 | MFS: -2.678 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1235600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1235600)
Modification: Disrupted | RMgm-3350
More info: PhenoPlasm Link
AlphaFill Uniprot ID: P50250
"Best" AlphaFill ligand hit: NAD (nicotinamide-adenine-dinucleotide, Local RMSD=0.09) with 3OND (Global RMSD=0.88)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.3.1.1 | adenosylhomocysteinase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P50247 | Adenosylhomocysteinase | Mus musculus | HOGENOM, OMA, OrthoDB | CHEMBL299135CHEMBL327105CHEMBL8275Neplanocin A... |
| P23526 | Adenosylhomocysteinase | Homo sapiens | HOGENOM, OMA, OrthoDB | CHEMBL296392CHEMBL301499CHEMBL299999CHEMBL50728... |
| P50250 | Adenosylhomocysteinase | HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | CHEMBL129014CHEMBL129469CHEMBL129014CHEMBL261619... | |
| P10760 | Adenosylhomocysteinase | Rattus norvegicus | HOGENOM, OMA, OrthoDB | CHEMBL2069133CHEMBL609353CHEMBL11390CHEMBL49204... |
| O43865 | S-adenosylhomocysteine hydrolase-like protein 1 | HOGENOM, OrthoDB | CHEMBL2059155CHEMBL3753637CHEMBL3753746 | |
| G1SVH0 | Adenosylhomocysteinase | OMA | US10227373, Compound 3-DeazaneplanocinUS10227373, Compound D-IsoneplanocinUS10227373, Compound L-IsoneplanocinCHEMBL154745... |
Ortholog Group (OrthoMCL): OG6_100715
Most Similar Human Ortholog: P23526
TM-align score: 0.98 | RMSD: 0.98
Seq Identity: 0.56 | Length: 431 / 479
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000101444 | adenosylhomocysteinase |
Protein Length: 479 | Molecular Weight (kDa): 53.839
UniProt ID(s): P50250
PDB ID(s): 1V8B
Isoelectric Point: 5.71
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000043 | Adenosylhomocysteinase-like |
| InterPro | IPR015878 | S-adenosyl-L-homocysteine hydrolase, NAD binding domain |
| InterPro | IPR020082 | S-adenosyl-L-homocysteine hydrolase, conserved site |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF00670 | S-adenosyl-L-homocysteine hydrolase, NAD binding domain |
| PFam | PF05221 | Adenosylhomocysteinase-like |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| Superfamily | SSF52283 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 23 | 12 | 22 |
| disruptive | 1 | 12 | 16 | 28 |
| missense | 1 | 12 | 16 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 40 | 17 | 25 |
| disruptive | 4 | 38 | 31 | 85 |
| missense | 3 | 29 | 27 | 57 |
PlasmoDB Total SNPs: 132
Non-coding: 116 | Synonymous: 12 | Nonsynonymous: 3 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 12910461 | Structure, evolution, and inhibitor interaction of S-adenosyl-L-homocysteinehydrolase from Plasmodium falciparum. | Bujnicki JM, Prigge ST, Caridha D, Chiang PK | 10.1002/prot.10446 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17150588 | Three-dimensional structure of S-adenosyl-L-homocysteine hydrolase fromPlasmodium falciparum. | Tanaka N, Nakanishi M, ..., Nakamura KT | 10.1093/nass/48.1.281 |
| 27503796 | Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. | Siau A, Huang X, ..., Preiser PR | 10.1038/srep31055 |
| 31537845 | The interactome of 2-Cys peroxiredoxins in Plasmodium falciparum. | Brandstaedter C, Delahunty C, ..., Becker K | 10.1038/s41598-019-49841-3 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |