PF3D7_0521500

Genome location: Pf3D7_05_v3:877,768..879,984(+)

Genome classification: Core

Function and Localization

Product Description: ribosomal large subunit pseudouridylate synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 4.2.1.70 (Pseudouridylate synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0009982	pseudouridine synthase activity
Function	GO:0004730	pseudouridylate synthase activity
Process	GO:0000455	enzyme-directed rRNA pseudouridine synthesis
Process	GO:0006364	rRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.08	0.96
Trophozoite	expressed	0.36	0.78
Schizont	expressed	0.04	0.97
Gametocyte	expressed	0.24	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0521500

Resistome Missense Mutations: E97G

Resistome Compounds with Missense Mutations: MMV006901

Resistome # Samples with Disruptive Mutations: 3 (3 missense, 3 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.016 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1111000)

Relative Growth Rate: 0.91 ± 0.16
Confidence: 5.05

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3P7

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.1.70	pseudouridylate synthase	tRNAiodoacetamide5-fluorouracilthiol reagents1,10-phenanthrolinep-chloromercuribenzoate1-(Tetrahydro-2-furanyl)-5-fluorouracil

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100211

Most Similar Human Ortholog: H3BRG3

TM-align score: 0.81 | RMSD: 1.97

Seq Identity: 0.26 | Length: 151 / 487

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000007376	RNA pseudouridine synthase domain containing 1
ENSG00000166133	RNA pseudouridine synthase domain containing 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	21	11	41
disruptive	7	35	34	73
missense	6	35	33	64

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	44	22	32
disruptive	13	105	56	128
missense	8	92	45	100

PlasmoDB Total SNPs: 111

Non-coding: 74 | Synonymous: 30 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 487 | Molecular Weight (kDa): 58.195

UniProt ID(s): Q8I3P7

PDB ID(s): None

Isoelectric Point: 9.21

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006145	Pseudouridine synthase, RsuA/RluA
InterPro	IPR020103	Pseudouridine synthase, catalytic domain superfamily
PFam	PF00849	Pseudouridine synthase, RsuA/RluA
Superfamily	SSF55120	Pseudouridine synthase, catalytic domain superfamily