PF3D7_0521700 (DDX1)

Genome location: Pf3D7_05_v3:882,262..885,355(+)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DDX1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Process	GO:0006364	rRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.12	0.94
Trophozoite	expressed	0.15	0.91
Schizont	expressed	0.02	0.98
Gametocyte	possibly expressed	0.14	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0521700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.064 | #Insertions: 7

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3P6

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.01) with 6L5M (Global RMSD=5.19)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_105046

Most Similar Human Ortholog: Q92499

TM-align score: 0.66 | RMSD: 4.24

Seq Identity: 0.31 | Length: 557 / 841

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000079785	DEAD-box helicase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	30	32	43
disruptive	16	64	35	113
missense	12	63	33	98

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	16	74	30	40
disruptive	27	133	76	173
missense	19	113	68	132

PlasmoDB Total SNPs: 81

Non-coding: 25 | Synonymous: 39 | Nonsynonymous: 17 | Stop Codon: 0

Protein Information

Protein Length: 841 | Molecular Weight (kDa): 97.338

UniProt ID(s): A7U5W9, Q8I3P6

PDB ID(s): None

Isoelectric Point: 7.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000629	ATP-dependent RNA helicase DEAD-box, conserved site
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR003877	SPRY domain
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR013320	Concanavalin A-like lectin/glucanase domain superfamily
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF00622	SPRY domain
Superfamily	SSF49899	Concanavalin A-like lectin/glucanase domain superfamily
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase