PF3D7_0522300

Genome location: Pf3D7_05_v3:902,269..905,399(-)

Genome classification: Core

Function and Localization

Product Description: 18S rRNA (guanine-N(7))-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.12 (Methionine S-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
Function	GO:0016435	rRNA (guanine) methyltransferase activity
Process	GO:0070476	rRNA (guanine-N7)-methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.41	0.79
Trophozoite	expressed	0.32	0.81
Schizont	expressed	0.06	0.96
Gametocyte	expressed	0.17	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0522300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.178 | MFS: -2.456 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4F7

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.22) with 4QTU (Global RMSD=1.19)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.12	methionine S-methyltransferase	NEMD-MetGly-MetL-ethionineL-methioninolN-formyl-L-MetL-MethioninamideL-Met ethyl esterL-Met methyl esterseleno-L-methionineL-Met p-nitroanilideS-methyl-L-methionine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102190

Most Similar Human Ortholog: O43709

TM-align score: 0.74 | RMSD: 1.04

Seq Identity: 0.56 | Length: 206 / 274

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000071462	BUD23 rRNA methyltransferase and ribosome maturation factor

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	17	12	19
disruptive	2	14	10	23
missense	2	14	9	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	33	16	16
disruptive	9	38	29	67
missense	7	30	25	51

PlasmoDB Total SNPs: 157

Non-coding: 142 | Synonymous: 13 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 274 | Molecular Weight (kDa): 31.593

UniProt ID(s): C0H4F7

PDB ID(s): None

Isoelectric Point: 9.9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR013216	Methyltransferase type 11
InterPro	IPR022238	18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23, C-terminal
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR039769	18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23-like
PFam	PF08241	Methyltransferase type 11
PFam	PF12589	18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23, C-terminal
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase