Genome location: Pf3D7_05_v3:995,782..1,002,522(+)
Genome classification: Core
Product Description: karyopherin beta
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0036396 | RNA N6-methyladenosine methyltransferase complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005643 | nuclear pore |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0061608 | nuclear import signal receptor activity |
| Function | GO:0008139 | nuclear localization sequence binding |
| Function | GO:0019904 | protein domain specific binding |
| Function | GO:0046982 | protein heterodimerization activity |
| Process | GO:0006607 | NLS-bearing protein import into nucleus |
| Process | GO:0006913 | nucleocytoplasmic transport |
| Process | GO:0006606 | protein import into nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.34 | 0.43 |
| Trophozoite | expressed | 1.08 | 0.45 |
| Schizont | expressed | 0.45 | 0.73 |
| Gametocyte | expressed | 0.53 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0524000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.14 | MFS: -2.943 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1238800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1238800)
Modification: Disrupted | RMgm-3362
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3M5
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101397
Most Similar Human Ortholog: H0Y3V4
TM-align score: 0.85 | RMSD: 1.86
Seq Identity: 0.21 | Length: 145 / 1123
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000065150 | importin 5 |
| ENSG00000137040 | RAN binding protein 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 51 | 20 | 55 |
| disruptive | 0 | 8 | 12 | 49 |
| missense | 0 | 8 | 11 | 41 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 133 | 35 | 60 |
| disruptive | 1 | 84 | 57 | 186 |
| missense | 0 | 55 | 41 | 103 |
PlasmoDB Total SNPs: 123
Non-coding: 99 | Synonymous: 23 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 1123 | Molecular Weight (kDa): 127.353
UniProt ID(s): O61140, Q7KAU3, Q8I3M5, Q9BN26
PDB ID(s): None
Isoelectric Point: 4.54
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR016024 | Armadillo-type fold |
| InterPro | IPR040122 | Importin beta family |
| InterPro | IPR041389 | Importin repeat 6 |
| InterPro | IPR041653 | Importin repeat 4 |
| PFam | PF12755 | Importin repeat 4 |
| PFam | PF18808 | Importin repeat 6 |
| PFam | PF18829 | |
| Superfamily | SSF48371 | Armadillo-type fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 12672529 | Cloning and characterization of Plasmodium falciparum homologs of nuclear importfactors, karyopherin alpha and karyopherin beta. | Mohmmed A, Kishore S, ..., Chauhan VS | 10.1016/s0166-6851(02)00331-6 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31384004 | Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. | Baumgarten S, Bryant JM, ..., Scherf A | 10.1038/s41564-019-0521-7 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |