Genome location: Pf3D7_05_v3:1,013,254..1,015,395(-)
Genome classification: Core
Product Description: ribosome-interacting GTPase 1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.3 (Threonine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.35 | 0.82 |
| Trophozoite | expressed | 0.31 | 0.81 |
| Schizont | expressed | 0.10 | 0.93 |
| Gametocyte | expressed | 0.26 | 0.84 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0524400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.123 | MFS: -2.942 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K870
"Best" AlphaFill ligand hit: ACP (adenosine-5'-[beta, gamma-methylene]triphosphate, Local RMSD=0.45) with 2OHF (Global RMSD=8.77)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.3 | threonine-tRNA ligase | borrelidinthreonyl-AMPZINC27215482ZINC67910544ZINC72320615ZINC72320626ZINC35270978ZINC35458951hydrogen peroxideoperator mRNA domain 2tubercidin 5'-triphosphatePurineriboside 5'-triphosphate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102018
Most Similar Human Ortholog: Q9Y295
TM-align score: 0.98 | RMSD: 1.01
Seq Identity: 0.71 | Length: 365 / 365
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000185721 | developmentally regulated GTP binding protein 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 19 | 13 | 24 |
| disruptive | 0 | 3 | 3 | 9 |
| missense | 0 | 3 | 2 | 5 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 44 | 12 | 16 |
| disruptive | 2 | 13 | 15 | 50 |
| missense | 0 | 6 | 10 | 35 |
PlasmoDB Total SNPs: 100
Non-coding: 95 | Synonymous: 4 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 365 | Molecular Weight (kDa): 40.685
UniProt ID(s): A0A0C5D0X8, A0A5K1K870
PDB ID(s): None
Isoelectric Point: 9.42
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004095 | TGS |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR006073 | GTP binding domain |
| InterPro | IPR006074 | GTP1/OBG, conserved site |
| InterPro | IPR012676 | TGS-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031662 | GTP binding protein, second domain |
| PFam | PF01926 | GTP binding domain |
| PFam | PF02824 | TGS |
| PFam | PF16897 | GTP binding protein, second domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF81271 | TGS-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 34706231 | Conserved heterodimeric GTPase Rbg1/Tma46 promotes efficient translation ineukaryotic cells | Zeng F, Li X, ..., Jin H | 10.1016/j.celrep.2021.109877 |
| 34900236 | Ribo-Seq and RNA-Seq of TMA46 ( DFRP1) and GIR2 ( DFRP2) knockout yeast strains | Egorov AA, Makeeva DS, ..., Dmitriev SE | 10.12688/f1000research.74727.1 |
| 19448108 | Saccharomyces cerevisiae Rbg1 protein and its binding partner Gir2 interact onPolyribosomes with Gcn1 | Wout PK, Sattlegger E, Sullivan SM, Maddock JR | 10.1128/EC.00356-08 |