Genome location: Pf3D7_05_v3:1,040,922..1,043,921(+)
Genome classification: Core
Product Description: acyl-CoA synthetase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0016020 | membrane |
| Function | GO:0003723 | RNA binding |
| Function | GO:0004467 | long-chain fatty acid-CoA ligase activity |
| Process | GO:0006631 | fatty acid metabolic process |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.55 | 0.37 |
| Trophozoite | expressed | 0.97 | 0.49 |
| Schizont | expressed | 0.60 | 0.66 |
| Gametocyte | expressed | 0.48 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0525100
Resistome Missense Mutations: M300I
Resistome Compounds with Missense Mutations: MMV665924
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.96 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3L4
"Best" AlphaFill ligand hit: SRT (s,r meso-tartaric acid, Local RMSD=0.16) with 5IE0 (Global RMSD=4.66)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100152
Most Similar Human Ortholog: H0Y9U7
TM-align score: 0.95 | RMSD: 1.52
Seq Identity: 0.42 | Length: 293 / 673
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151726 | acyl-CoA synthetase long chain family member 1 |
| ENSG00000164398 | acyl-CoA synthetase long chain family member 6 |
| ENSG00000197142 | acyl-CoA synthetase long chain family member 5 |
| ENSG00000281938 | novel transcript |
Protein Length: 673 | Molecular Weight (kDa): 76.852
UniProt ID(s): Q8I3L4
PDB ID(s): None
Isoelectric Point: 8.28
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR020845 | AMP-binding, conserved site |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| Superfamily | SSF56801 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 22 | 11 | 39 |
| disruptive | 25 | 19 | 17 | 41 |
| missense | 25 | 19 | 16 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 48 | 26 | 45 |
| disruptive | 29 | 52 | 32 | 118 |
| missense | 28 | 43 | 29 | 64 |
PlasmoDB Total SNPs: 164
Non-coding: 114 | Synonymous: 24 | Nonsynonymous: 26 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 29326268 | Mapping the malaria parasite druggable genome by using in vitro evolution andchemogenomics. | Cowell AN, Istvan ES, ..., Winzeler EA | 10.1126/science.aan4472 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |