Genome location: Pf3D7_05_v3:1,124,729..1,129,071(-)
Genome classification: Core
Product Description: DNA replication licensing factor MCM3, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.3.3.6 (Acyl-CoA oxidase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042555 | MCM complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003688 | DNA replication origin binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0006271 | DNA strand elongation involved in DNA replication |
| Process | GO:0000727 | double-strand break repair via break-induced replication |
| Process | GO:1902975 | mitotic DNA replication initiation |
| Process | GO:0006267 | pre-replicative complex assembly involved in nuclear cell cycle DNA replication |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.88 | 0.54 |
| Schizont | expressed | 0.73 | 0.61 |
| Gametocyte | expressed | 0.89 | 0.55 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0527000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.418 | MFS: -2.514 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1241800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3J5
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.20) with 4R7Y (Global RMSD=3.67)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.3.3.6 | acyl-CoA oxidase | KICoAFMNKClKCNKBrKNO3HgCl2NH4ClAgNO3phenolNH4SCN... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101598
Most Similar Human Ortholog: P25205
TM-align score: 0.76 | RMSD: 4.12
Seq Identity: 0.38 | Length: 695 / 962
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000112118 | minichromosome maintenance complex component 3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 58 | 27 | 80 |
| disruptive | 26 | 48 | 36 | 62 |
| missense | 10 | 29 | 29 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 150 | 35 | 53 |
| disruptive | 42 | 106 | 44 | 107 |
| missense | 16 | 73 | 32 | 78 |
PlasmoDB Total SNPs: 210
Non-coding: 158 | Synonymous: 18 | Nonsynonymous: 34 | Stop Codon: 0
Protein Length: 962 | Molecular Weight (kDa): 109.798
UniProt ID(s): Q8I3J5
PDB ID(s): None
Isoelectric Point: 5.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001208 | MCM domain |
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR008046 | DNA replication licensing factor Mcm3 |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031327 | Mini-chromosome maintenance protein |
| InterPro | IPR033762 | MCM OB domain |
| InterPro | IPR041562 | MCM, AAA-lid domain |
| PFam | PF00493 | MCM domain |
| PFam | PF17207 | MCM OB domain |
| PFam | PF17855 | MCM, AAA-lid domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |