Genome location: Pf3D7_05_v3:1,129,570..1,131,702(-)
Genome classification: Core
Product Description: ubiquitin-conjugating enzyme E2 13
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.23 (E2 ubiquitin-conjugating enzyme);6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0006301 | postreplication repair |
| Process | GO:0070534 | protein K63-linked ubiquitination |
| Process | GO:0000209 | protein polyubiquitination |
| Process | GO:0016567 | protein ubiquitination |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.11 | 0.93 |
| Schizont | expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0527100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.146 | MFS: -3.121 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1241900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1241900)
Modification: Tagged | RMgm-5205
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3J4
"Best" AlphaFill ligand hit: BU3 ((r,r)-2,3-butanediol, Local RMSD=0.04) with 1Z2U (Global RMSD=0.94)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.23 | E2 ubiquitin-conjugating enzyme | CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione... |
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P61088 | Ubiquitin-conjugating enzyme E2 N | Homo sapiens | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | MLS000074439MLS000032400MLS000041097MLS000055488... |
| P63146 | Ubiquitin-conjugating enzyme E2 B | OrthoDB | CHEMBL3787660 | |
| P51965 | Ubiquitin-conjugating enzyme E2 E1 | OrthoDB | US9796725, Compound I-001US9796725, Compound I-098US9796725, Compound I-003US9796725, Compound I-004... |
Ortholog Group (OrthoMCL): OG6_101934
Most Similar Human Ortholog: P61088
TM-align score: 0.98 | RMSD: 0.48
Seq Identity: 0.67 | Length: 151 / 152
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000177889 | ubiquitin conjugating enzyme E2 N |
Protein Length: 152 | Molecular Weight (kDa): 17.411
UniProt ID(s): Q8I3J4
Isoelectric Point: 6.87
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000608 | Ubiquitin-conjugating enzyme E2 |
| InterPro | IPR016135 | Ubiquitin-conjugating enzyme/RWD-like |
| InterPro | IPR023313 | Ubiquitin-conjugating enzyme, active site |
| PFam | PF00179 | Ubiquitin-conjugating enzyme E2 |
| Superfamily | SSF54495 | Ubiquitin-conjugating enzyme/RWD-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 140
Non-coding: 138 | Synonymous: 2 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 34750454 | Deletion of Plasmodium falciparum ubc13 increases parasite sensitivity to themutagen, methyl methanesulfonate and dihydroartemisinin. | Maneekesorn S, Knuepfer E, ..., Holder AA | 10.1038/s41598-021-01267-6 |
| 35490588 | Protein kinase 9 is not required for completion of the Plasmodium berghei lifecycle. | Narwal SK, Nayak B, Mehra P, Mishra S | 10.1016/j.micres.2022.127051 |
| 36088506 | Mrz1, a Novel Mitochondrial Outer Membrane RING Finger Protein, is DegradedThrough the Ubiquitin-Proteasome Pathway in Schizosaccharomyces pombe | Liu Z, Zhang P, ..., Huang Y | 10.1007/s00284-022-02998-z |
| 37738679 | High-throughput screening of the Saccharomyces cerevisiae genome for2-amino-3-methylimidazo [4,5-f] quinoline resistance identifies coloncancer-associated genes | Dolan M, St John N, ..., Fasullo M | 10.1093/g3journal/jkad219 |